CNOT10 Antibody (PCRP-CNOT10-1D5) - Azide and BSA Free

Images

 
Protein Array: CNOT10 Antibody (PCRP-CNOT10-1D5) - Azide and BSA Free [NBP3-14266] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CNOT10 Antibody (PCRP-CNOT10-1D5).
Immunofluorescence Analysis of PFA-fixed HeLa cells stained using CNOT10 Antibody (PCRP-CNOT10-1D5) - Azide and BSA Free
Flow cytometric analysis of PFA-fixed HeLa cells. CNOT10 Antibody (PCRP-CNOT10-1D5) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, MA
Clone
PCRP-CNOT10-1D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

CNOT10 Antibody (PCRP-CNOT10-1D5) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13921).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human CNOT10 protein (Uniprot: Q9H9A5)
Localization
Cytoplasm, Mitochondrion and Nucleus, PML body
Isotype
IgG2c
Clonality
Monoclonal
Host
Mouse
Gene
CNOT10
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Theoretical MW
82.31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CNOT10 Antibody (PCRP-CNOT10-1D5) - Azide and BSA Free

  • CCR4-NOT transcription complex subunit 10
  • CCR4-NOT transcription complex, subunit 10
  • DKFZp434K115
  • FLJ12890
  • FLJ13165

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CNOT10