Chromata ChIP Histone H3 [Trimethyl Lys4] Kit (ChIP)

Product Discontinued

Chromata ChIP Histone H3 [Trimethyl Lys4] Kit (ChIP) Summary

• Description: This ChIP kit is specific for Histone H3 trimethylated Lysine 4, and contains all the necessary components to perform 25 Immunoprecipitation experiments and a positive control antibody (NB21-1023SS). This ChIP kit is a complete pack to help you perfom Chromatin Immunoprecipitation efficiently and accurately.
• Modification: Trimethyl Lys4
• Kit Type: Kit (ChIP)

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

Click here for detailed information on the ChromataChIP kit. View the complete ChromatChIP Kit components and protocol here.

Alternate Names for Chromata ChIP Histone H3 [Trimethyl Lys4] Kit (ChIP)

• H3K4Me3

Background

Novus' ChromataChIP Kits are a complete pack to help you perform Chromatin Immunoprecipitation efficiently and accurately in your laboratory. We've taken all the guesswork out of ChIP by testing multiple component options to find the best possible component for you, the customer. ChromataChIP Kits come with everything you need to perform Chromatin IP including a colorful, step-by-step protocol pamphlet to walk you through the 4 main phases of ChIP. Learn more by visiting www.novusbio.com/ChromataChIP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Chromata ChIP Histone H3 Kit (NBP1-71716)(2)

Publications using NBP1-71716

• Ushio A, Eto K. RBM3 expression is upregulated by NF-kB p65 activity, protecting cells from apoptosis, during mild hypothermia. J. Cell. Biochem. 2018-02-01 [PMID: 29388696] (Chemotaxis)
• Noto PB, Bukhtiyarov Y, Meng S et al. Regulation of Sphingomyelin Phosphodiesterase, Acid-like 3A Gene (SMPDL3A) by Liver X Receptors Mol Pharmacol 2012-07-24 [PMID: 22810003] (Chemotaxis)

FAQs for Chromata ChIP Histone H3 Kit (NBP1-71716). (Showing 1 - 1 of 1 FAQs).

1. I have one question about ChIP. In your Chromata ChIP kit, you add 25ul magnetic beads to the samples. Is there any way to normalize for differences in the amounts of magnetic beads in each sample? For example, it can happen that one sample got a bit more beads than another, is there some way to take this into account when you analyze your data?
   • As long as the vial/bottle of beads is vortexed so that the beads are fully resuspended in solution before taking the 25ul out, there will not be any significant variation in bead number. However, if you are taking multiple 25ul aliquots out, it is best to quickly revortex before every aliquot pipetting. For all of our internal testing, we typically see a CV of less than 2% for both intra- and interassay variation, which is very good.