ChromataChIP Kits

Novus’ ChromataChIP™ Kits are a complete ChIP solution to help you perform chromatin immunoprecipitation efficiently and accurately in your laboratory. We have optimized this kit and have selected only the best components for this kit.

Why choose the ChromataChIP™ kit?

Easy to use – ChromataChIP™ is easy to follow. Our straightforward protocol will allow you to start chromatin immunoprecipitations immediately.

Saves money – ChromataChIP™ kits are more cost effective than buying and testing separate components. In addition, all of our components have been individually tested to be the best in class. These critical components include:

  • Magnetic Protein A/G beads – With magnetic beads, you save time by performing quick washes and save money by using less antibody with each IP. Agarose bead users must first pre-clear and block the beads in order to minimize background, which is unnecessary with magnetic beads. Agarose also adds time to every wash because users must separate the beads from washing buffers without pipetting up any of the beads or leaving behind any excess wash. Magnetic beads require no blocking, no centrifugation, and beads completely separate from buffers with the aid of higher recovery thus providing less antibody with magnetic beads, allowing you to conserve antibody.
  • Positive control antibody and primers – We include a vial of our Epi-plus H3 K4Me3 antibody with every kit. This, along with a human RPL30 primer set, will take the guess work out of ChIP.
  • DNA purification columns – These best in class columns, consistently give you maximum recovery in under 10 minutes, without messy phenol/chloroform cleanup.
ChromataChip Stages
  Agarose Beads Magnetic Beads
Preclear and Block Beads Required Not Required
Background Signal High Low
False Positives High Low
Protocol Time Longer Shorter
Amount of Antibody Used More Less
Can be Automated No Yes

Customer Feedback

"I have been very impressed with Novus Biologicals in general, not only the ChIP kit but the company as a whole. The customer service is very quick and helpful, and always answers the queries even if they need to be forwarded to someone else. I especially like the possibility to chat with research and sales advisors on the website which no other company to my knowledge has, and which has saved my neck a few times....Was there anything that was above your expectations? The kit was very easy to follow. I was really struggling with [another company's] kit, but the ChromataChIP is efficient, the protocol is very easy, and it works!"

"I am a big fan of Novus and have recommended it to all my colleagues. I work with chromatin IP and received tremendous support from Novus to get it working. Also the Chromata ChIP kit is the best on the market."

ChromataChIP Kit Components

Novus' ChromataChIP™ Kits come with the key components you need to perform Chromatin Immunoprecipitation, including a colorful, step-by-step protocol pamphlet to walk you through the 5 main phases of ChIP. Download the colorful ChromataChIP kit protocol booklet.
 
• Positive Control Antibody (H3K4Me3)
• Protein A/G Magnetic Beads
• DNA Purification Kit
• Positive Control Primers (RPL30)
• 10X Glycine
• IP Dilution Buffer
• IP Wash Buffer 1
• IP Wash Buffer 2
• IP Wash Buffer 3
• IP Wash Buffer 4
• IP Elution Buffer
• NaCl solution
• Proteinase K

ChIP Image

ChIP Image

Optimally sheared chromatin, lane 3.

 
Novus ChromataChIP
Company A
Company M
Company I
Magnetic Beads
YES
YES
NO
YES
DNA Purification Kit
YES
NO
YES
YES
Epigenetic Positive Control Antibody
YES
NO
NO
NO
Positive Control PCR Primer Set
YES
NO
YES
NO
Simple Protocol
YES
NO
NO
NO
Less Than 2 Hours Hands On Time
YES
NO
NO
NO

Available Kits

Novus ChromataChIP kits
Description
Basic 25 IP kit
25 IP kit + H3 ac Lys4
25 IP kit + H3 Me3 Lys9
25 IP kit + H3 Me2 Lys9
25 IP kit + H3 Me1 Lys9
25 IP kit + H3 ac Lys9/ac Lys14

 

Learn more about Chromatin immunoprecipitation and ChromataChIP™ Kits by watching this webinar
Chip Webinar

Related Components

 

Chip Image of H3 K4Me3 RPL30 Primer RPL30 Primer
Histone H3 Antibody
2 ug of NB21-1023 was used to IP
DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
RPL30 Primer
qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
RPL30 Primer
PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.

 

Related Products

Epi-Plus AntibodiesChIP-Validated AntibodiesPrecipitorPrimers