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ChromataChIP Kits
Novus’ ChromataChIP™ Kits are a complete ChIP solution to help you perform chromatin immunoprecipitation efficiently and accurately in your laboratory. We’ve taken all the guesswork out of ChIP by testing multiple component options to make the best kit on the market.

Why is the ChromataChip kit the best?

Simple to use – ChromataChIP™ is the simplest kit on the market. Our straightforward protocol will allow you to start chromatin immunoprecipitations immediately instead of fumbling throughan overly complicated handbook.

Saves money – ChromataChIP™ kits are more cost effective than buying and testing separate components. In addition, all of our components have been individually tested to be the best in class. Since it only takes one component’s failure to ruin an entire assay, our kit eliminates this variable. These critical components include:

  • Magnetic Protein A/G beads – With magnetic beads, you save time by performing quicker washes and save money by using less antibody with each IP. Agarose bead users must first pre-clear and block the beads in order to minimize background, which is unnecessary with magnetic beads. Agarose also adds time to every wash because users much separate the beads from washing buffers without pipetting up any of the beads or leaving behind any excess wash, a nearly impossible feat! With magnetic beads, there is no blocking, no centrifugation, and beads completely separate from buffers with the aid of a magnet. In addition, you use 2-5 times less antibody with magnetic beads, making your precious antibody last much longer.

  • Positive control antibody and primers – We include a vial of our Epi-plus H3 K4Me3 antibody with every kit. This, along with a human RPL30 primer set, will take the guess work out of ChIP.
  • DNA purification columns – These best in class columns consistently give you maximum recovery in under 10 minutes without messy phenol/chloroform cleanup.
  Agarose Beads Magnetic Beads
Preclear and Block Beads Required Not Required
Background Signal High Low
False Positives High Low
Protocol Time Longer Shorter
Amount of Antibody Used More Less
Can be Automated No Yes


ChromataChIP Kit Components

Novus' ChromataChIP™ Kits come with the key components you need to perform Chromatin Immunoprecipitation, including a colorful, step-by-step protocol pamphlet to walk you through the 5 main phases of ChIP. Download the pamphlet here
 
• Positive Control Antibody  (H3K4Me3)
• Protein A/G Magnetic Beads
• DNA Purification Kit
• Positive Control Primers (RPL30)
• 10X Glycine
• IP Dilution Buffer
• IP Wash Buffer 1
• IP Wash Buffer 2
• IP Wash Buffer 3
• IP Wash Buffer 4
• IP Elution Buffer
• NaCl solution
• Proteinase K


Optimally sheared chromatin, lane 3.  

 
Novus ChromataChIP
Company A
Company M
Company I
Magnetic Beads
YES
YES
NO
YES
DNA Purification Kit
YES
NO
YES
YES
Epigenetic Positive Control Antibody
YES
NO
NO
NO
Positive Control PCR Primer Set
YES
NO
YES
NO
Simple Protocol
YES
NO
NO
NO
Less Than 2 Hours Hands On Time
YES
NO
NO
NO


Available Kits

Novus ChromataChIP kits
Description
Price (US)
Price (CAN)
Price (Euro)
Basic 25 IP kit 
$440
C$495
€393
25 IP kit + H3 ac Lys4
$575
C$647
€513
25 IP kit + H3 Me3 Lys9
$575
C$647
€513
25 IP kit + H3 Me2 Lys9
$575
C$647
€513
25 IP kit + H3 Me1 Lys9
$575
C$647
€513
25 IP kit + H3 ac  Lys9/ac Lys14
$575
C$647
€513

 

 

 

 

 

 

 


Learn more about Chromatin immunoprecipitation and ChromataChIP™ Kits by watching this webinar

 

Related Components

Product Name
Catalog #
Price ($USD)
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Price (£)
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£70.00 
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$731.00 
£450.00

 

 

Chip Image of H3 K4Me3

Histone H3 Antibody

2 ug of NB21-1023 was used to IP
DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).

RPL30 Primer

qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.

RPL30 Primer

PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.

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