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Novus’ ChromataChIP™ Kits are a complete ChIP solution to help you perform chromatin immunoprecipitation efficiently and accurately in your laboratory. We have optimized this kit and have selected only the best components for this kit.
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Why choose the ChromataChIP™ kit?
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Easy to use – ChromataChIP™ is easy to follow. Our straightforward protocol will allow you to start chromatin immunoprecipitations immediately.
Saves money – ChromataChIP™ kits are more cost effective than buying and testing separate components. In addition, all of our components have been individually tested to be the best in class. These critical components include:
- Magnetic Protein A/G beads – With magnetic beads, you save time by performing quick washes and save money by using less antibody with each IP. Agarose bead users must first pre-clear and block the beads in order to minimize background, which is unnecessary with magnetic beads. Agarose also adds time to every wash because users must separate the beads from washing buffers without pipetting up any of the beads or leaving behind any excess wash. Magnetic beads require no blocking, no centrifugation, and beads completely separate from buffers with the aid of higher recovery thus providing less antibody with magnetic beads, allowing you to conserve antibody.
- Positive control antibody and primers – We include a vial of our Epi-plus H3 K4Me3 antibody with every kit. This, along with a human RPL30 primer set, will take the guess work out of ChIP.
- DNA purification columns – These best in class columns, consistently give you maximum recovery in under 10 minutes, without messy phenol/chloroform cleanup.
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Agarose Beads |
Magnetic Beads |
| Preclear and Block Beads |
Required |
Not Required |
| Background Signal |
High |
Low |
| False Positives |
High |
Low |
| Protocol Time |
Longer |
Shorter |
| Amount of Antibody Used |
More |
Less |
| Can be Automated |
No |
Yes |
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Customer Feedback
"I have been very impressed with Novus Biologicals in general, not only the ChIP kit but the company as a whole. The customer service is very quick and helpful, and always answers the queries even if they need to be forwarded to someone else. I especially like the possibility to chat with research and sales advisors on the website which no other company to my knowledge has, and which has saved my neck a few times....Was there anything that was above your expectations? The kit was very easy to follow. I was really struggling with [another company's] kit, but the ChromataChIP is efficient, the protocol is very easy, and it works!"
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ChromataChIP Kit Components
| Novus' ChromataChIP™ Kits come with the key components you need to perform Chromatin Immunoprecipitation, including a colorful, step-by-step protocol pamphlet to walk you through the 5 main phases of ChIP. Download the colorful ChromataChIP kit protocol booklet. |
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| • Positive Control Antibody (H3K4Me3) |
| • Protein A/G Magnetic Beads |
| • DNA Purification Kit |
| • Positive Control Primers (RPL30) |
| • 10X Glycine |
| • IP Dilution Buffer |
| • IP Wash Buffer 1 |
| • IP Wash Buffer 2 |
| • IP Wash Buffer 3 |
| • IP Wash Buffer 4 |
| • IP Elution Buffer |
| • NaCl solution |
| • Proteinase K |
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Optimally sheared chromatin, lane 3.
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Novus ChromataChIP
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Company A
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Company M
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Company I
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Magnetic Beads
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YES
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YES
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NO
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YES
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DNA Purification Kit
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YES
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NO
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YES
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YES
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Epigenetic Positive Control Antibody
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YES
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NO
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NO
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NO
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Positive Control PCR Primer Set
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YES
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NO
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YES
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NO
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Simple Protocol
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YES
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NO
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NO
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NO
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Less Than 2 Hours Hands On Time
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YES
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NO
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NO
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NO
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Available Kits
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Novus ChromataChIP kits
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Description
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Basic 25 IP kit
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25 IP kit + H3 ac Lys4
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25 IP kit + H3 Me3 Lys9
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25 IP kit + H3 Me2 Lys9
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25 IP kit + H3 Me1 Lys9
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25 IP kit + H3 ac Lys9/ac Lys14
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Learn more about Chromatin immunoprecipitation and ChromataChIP™ Kits by watching this webinar
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Related Components
Histone H3 Antibody
2 ug of NB21-1023 was used to IP
DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1). |
RPL30 Primer
qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin. |
RPL30 Primer
PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate. |
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