Cholesterol Oxidase Antibody

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Western Blot: Cholesterol Oxidase Antibody [NB600-625] - Detection of purified cholesterol oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified cholesterol oxidase contained 4% BME ...read more
Western Blot: Cholesterol Oxidase Antibody [NB600-625] - Analysis using HRP conjugate of NB600-625. Detection of purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

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Cholesterol Oxidase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Cholesterol Oxidase (Microorganism).
Specificity
Cholesterol Oxidase
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:5000
  • ELISA 1:5000 - 1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Cholesterol Oxidase [Microorganism] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:2,000 - 1:8,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reacts with bacteria.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Cholesterol Oxidase Antibody

  • choA
  • choD
  • choM
  • choS
  • COD

Background

Cholesterol Oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Cholesterol Oxidase Products

Cholesterol Oxidase NB600-625

Research Areas for Cholesterol Oxidase Antibody (NB600-625)

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