Chikungunya Virus E1 Antibody (988110) [Unconjugated]

Recombinant Viral Chikungunya Virus E1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97791) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB9779) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: V
• Applications: ELISA
• Clone: 988110
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Chikungunya Virus E1 Antibody (988110) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived transfected with viral Chikungunya Virus E1
  Tyr810-Gly1224
  Accession # Q8JUX5
• Specificity: Detects viral Chikungunya Virus E1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chikungunya Virus E1 Antibody (988110) [Unconjugated]

• CHICKV E1
• Chikungunya Virus E1
• Chikungunya Virus
• CHIKV-E1

Background

Chikungunya fever is a pandemic disease caused by the flavivirus Chikungunya (CHIKV). The 47 kDa E1 glycoprotein mediates viral membrane fusion during CHIKV infection. The CHIKV envelope protein E1 is a component of the viral spike, which is composed of triplets of heterodimer of E1 and E2 glycoproteins, expressed on the viral surface. The viral spike proteins facilitate attachment to cell surfaces and viral entry into the cells. The E1 envelope protein is a class II fusion protein that mediates low pH-triggered membrane fusion during virus infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8JUX5()