Chicken IgM Native Protein

SDS-Page: Chicken IgM Native Protein [NBP1-96785] - SDS-PAGE of Chicken IgM Whole Molecule. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.

• Reactivity: Ch
• Applications: WB, ELISA, IHC, PAGE

Chicken IgM Native Protein Summary

• Description: Native Chicken Chicken IgM
  
  Source: Normal serum
  
  This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
• Preparation Method: This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Chicken IgM whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum and anti-Chicken IgM (u chain specific). No reaction was observed against anti-Chicken IgG F(c). Some light chain cross reactivity will occur with anti-Chicken IgG. Analysis by SDS-PAGE was used to show purity at greater than 95%. Some high molecular weight banding may be visible. A minor band at ~50 kDa may also be visible.
• Protein/Peptide Type: Native Protein
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Preservative: 0.1% Sodium Azide
• Purity: Multi-step

Background

Immunoglobulin M is the largest antibody isotype and the first to be secrected against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approixmate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.