ChABC Antibody (1E10) - BSA Free

Western Blot: ChABC Antibody (1E10) [NBP1-96141] - (ChABC/1E10) and NBP1-96142 (ChABC/6A12). See Notes for additional information.

• Reactivity: Rt, All-NA
• Applications: WB, ELISA
• Clone: 1E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ChABC Antibody (1E10) - BSA Free Summary

• Immunogen: Chondroitinase ABC
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: This ChABC (1E10) antibody is useful for ELISA and Western blot, where a band can be seen at approximately 80 kDa. It can be used in Sandwich ELISA as the detection antibody with catalog # NBP1-96142, ChABC (6A12) as the capture antibody. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Western blot data: 125 ng Chondroitinase ABC was mixed with 100 ug of fetal bovine serum (as a background control). The proteins were mixed with 100 ul of non-reducing sample buffer and applied to a single 6 cm well set in a SDS-PAGE minigel (approx 20 ng/well [0.5 cm]). The gel bands were electroblotted to a nitrocellulose sheet. Immunolabeling for individual antibodies was performed on the sheet placed in a slot blot devise. Purified NBP1-96141 (ChABC/1E10) and NBP1-96142 (ChABC/6A12) were applied at 5 ug/ml IgG.
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27364797).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ChABC Antibody (1E10) - BSA Free

• Chondroitinase ABC

Background

ChABC (Chondroitinase ABC) is a bacterial enzyme that has been used for decades, mostly for biochemical characterization of chondroitin sulfate proteoglycans (CSPG). This enzyme degrades the sugar chains attached to the proteoglycan core protein. More recently, ChABC has been used to therapeutically degrade and inactivate CSPG in vivo. CSPGs are involved in a variety of biological processes and play a major inhibitory role in regeneration in the nervous system. For instance, CSPGs have been implicated in preventing regeneration after spinal cord injury. Injection of ChABC at the site of spinal cord injury has improved recovery in numerous animal models. ChABC antibodies serve as tools to help determine the ChABC distribution and content in tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ChABC Antibody (NBP1-96141)(4)

Publications using NBP1-96141

• Al-Nakouzi N, Wang CK, Oo HZ et al. Reformation of the chondroitin sulfate glycocalyx enables progression of AR-independent prostate cancer Nature communications 2022-08-13 [PMID: 35963852] (IHC-P, Human)
  
  Details:
  Supplementary Figure 9
• Lu T, Peng W, Liang Y et al. PTEN-silencing combined with ChABC-overexpression in adipose-derived stem cells promotes functional recovery of spinal cord injury in rats Biochemical and Biophysical Research Communications 2020-09-01 [PMID: 32888649] (WB, Rat)
• Gao J, Xia B, Li S et al. Magnetic Field Promotes Migration of Schwann Cells with Chondroitinase ABC (ChABC)-Loaded Superparamagnetic Nanoparticles Across Astrocyte Boundary in vitro Int J Nanomedicine 2020-01-20 [PMID: 32021182] (WB)
• Wu JH, Li M, Liang Y et al. Migration of Adipose-derived Mesenchymal Stem Cells Stably Expressing Chondroitinase ABC In vitro Chin. Med. J. 2016-07-01 [PMID: 27364797] (WB, Rat)

FAQs for ChABC Antibody (NBP1-96141). (Showing 1 - 1 of 1 FAQs).

1. Can you tell me antibodies NBP1-96142 and NBP1-96141 have been tested for use in IHC? I would like use them for cardiac samples from my mouse model.
   • I have taken a look into our data, and unfortunately, neither of the mentioned antibodies have been tested in IHC yet. We have only validated these in WB and ELISA, and while they may very well work in IHC, we cannot guarantee it.