Cell Viability Assay Kit (Fluorometric)

Product Discontinued

Cell Viability Assay Kit (Fluorometric) Summary

• Description: Easy, non-radioactive assay kit to measure cell viability/proliferation, cytotoxicity and apoptosis by microscopy/FACS (Flow cytomertry) analysis.
  
  Detection method: Fluorescent microscopy or FACS analysis Ex/Em = 485/530 nm to detect stained live cells and Ex/Em = 495/635 to detect stained dead cells. Applications: Screening/studying/characterization of stimulators/inhibitors that affect cell viability.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab287858)
• Kit Type: Assay Kit (Fluorometric)

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

Quantification of number of live and dead cells is an indispensable tool in cell biology research. The Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact, live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.