| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clone | A51-B/A6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13931). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Human breast cancer MCF-7 cells |
| Epitope | Fuca1-2 Galb1-4 GlcNAc |
| Localization | Cell Surface |
| Specificity | This antibody reacts with H type 2 blood group epitope Fuca1-2 Galb1-4 GlcNAc. It does not cross-react with human blood group H type 1, 3 or 4, nor with the closely related type 2 antigen Ley and Lex. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular trans- formation. Blood group related antigens are usually mucin-type and are detected on erythrocytes, certain epithelial cells and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A (1 and 2), B, H (1 and 2), M, N, Lewis A, Lewis B, Lewis X, Lewis Y and precursor type 1 chain antigens. The expressions of the H1 and H2 in different cell types are con- trolled by different genes. |
| Isotype | IgA |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | PEG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistology (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | PEG purified |
Secondary Antibodies |
Isotype Controls |
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