CCDC94 Antibody

Western Blot: CCDC94 Antibody [NBP2-78706] - Detection of human CCDC94 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, and MCF-7 cells prepared using NETN lysis buffer. This antibody was used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: CCDC94 Antibody [NBP2-78706] - Detection of human CCDC94 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: this antibody was used for IP at 100 ul per reaction. For blotting immunoprecipitated CCDC94, this antibody was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Product Discontinued

CCDC94 Antibody Summary

• Immunogen: Maps to a region between residue 273 and 323.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YJU2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 50 - 100 ul/mg lysate
  • Western Blot 1:1000
• Application Notes: Western blot of immunoprecipitates performed using Normal Pig Serum Goat, anti-Rabbit Light Chain HRP Conjugate, and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Tris-buffered Saline containing 0.1% BSA.
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for CCDC94 Antibody

• coiled-coil domain containing 94
• coiled-coil domain-containing protein 94
• FLJ10374

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: YJU2