![Western Blot: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CA19-9/Sialyl Lewis A antibody [GTX933] (NBP3-13650) diluted at 1:1000 and competitor's antibody diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT933-Western-Blot-NBP3-13650-img0006.jpg "Western Blot: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CA19-9/Sialyl Lewis A antibody [GTX933] (NBP3-13650) diluted at 1:1000 and competitor's antibody diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, MA |
| Clone | GT933 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | The immunogen used to generate this antibody corresponds to CA19-9/Sialyl Lewis A. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for CA19-9/Sialyl Lewis A Antibody (NBP3-13650)Find related products by research area.
|
|
Autophagy and RAS signaling: Clinical implications By Christina Towers, PhD The cellular recycling process known as autophagy is currently being targeted in over 60 clinical trials focused on treating different types of cancer1. To date, the only autophagy-targeted ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - CA19-9/Sialyl Lewis A antibody [GT933] detects CA19-9/Sialyl Lewis A protein at cell membrane by immunofluorescent analysis. Sample: SW1116 (high expression) and HT29 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CA19-9/Sialyl Lewis A stained by CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) diluted at 1:1000 and competitor's antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT933-Immunocytochemistry-Immunofluorescence-NBP3-13650-img0001.jpg "Immunocytochemistry/Immunofluorescence: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - CA19-9/Sialyl Lewis A antibody [GT933] detects CA19-9/Sialyl Lewis A protein at cell membrane by immunofluorescent analysis. Sample: SW1116 (high expression) and HT29 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CA19-9/Sialyl Lewis A stained by CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) diluted at 1:1000 and competitor's antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.")
![Flow Cytometry: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) detects CA19-9/Sialyl Lewis A by flow cytometry analysis. Sample: COLO 205 cell. Black: Unlabelled sample was used as a control. Red: CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) dilution: 1:50. Blue: Highly cited antibody [116-NS-19-9] dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT933-Flow-Cytometry-NBP3-13650-img0002.jpg "Flow Cytometry: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) detects CA19-9/Sialyl Lewis A by flow cytometry analysis. Sample: COLO 205 cell. Black: Unlabelled sample was used as a control. Red: CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) dilution: 1:50. Blue: Highly cited antibody [116-NS-19-9] dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.")
![ELISA: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Competition analysis of CA 19-9 antibody [GT933] (NBP3-13650) (330 ng/mL) binding to immobilized recombinant HSA-CA 19-9 (coated at 3 ug/mL) in the presence of soluble Lewis A or Lewis X tetrasaccharides (1.0-0.01 mM). Bound antibody was detected by Goat Anti-Mouse IgG antibody (HRP) (NBP2-19382) (1:10000).](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT933-ELISA-NBP3-13650-img0003.jpg "ELISA: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Competition analysis of CA 19-9 antibody [GT933] (NBP3-13650) (330 ng/mL) binding to immobilized recombinant HSA-CA 19-9 (coated at 3 ug/mL) in the presence of soluble Lewis A or Lewis X tetrasaccharides (1.0-0.01 mM). Bound antibody was detected by Goat Anti-Mouse IgG antibody (HRP) (NBP2-19382) (1:10000).")
![ELISA: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9/Sialyl Lewis A antibody [GTX933] (NBP3-13650) as capture antibody at concentration of 5 ug/mL and HRP-conjugated Ca19-9 antibody [GT8510] (NBP3-13652) as detection antibody at concentration of 1 ug/mL. Please notice that NBP3-13652 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13650. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT933-ELISA-NBP3-13650-img0004.jpg "ELISA: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9/Sialyl Lewis A antibody [GTX933] (NBP3-13650) as capture antibody at concentration of 5 ug/mL and HRP-conjugated Ca19-9 antibody [GT8510] (NBP3-13652) as detection antibody at concentration of 1 ug/mL. Please notice that NBP3-13652 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13650. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9/Sialyl Lewis A antibody [GT8510] (NBP3-13652) as capture antibody at concentration of 5 ug/mL and HRP-conjugated CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) as detection antibody at concentration of 1 ug/mL. Please notice that NBP3-13650 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13652. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT933-Sandwich-ELISA-NBP3-13650-img0005.jpg "Sandwich ELISA: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9/Sialyl Lewis A antibody [GT8510] (NBP3-13652) as capture antibody at concentration of 5 ug/mL and HRP-conjugated CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) as detection antibody at concentration of 1 ug/mL. Please notice that NBP3-13650 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13652. Please contact us for custom HRP-conjugated antibody.")
![Microarray: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - CA19-9/Sialyl Lewis A antibody [GT933] specifically detects sialyl-Lewis A (Neu5Ac-; CA19-9/Sialyl Lewis A) on a glycan array. Green: sialyl-Lewis A (Neu5Ac-; CA19-9/Sialyl Lewis A). Red: Modified sialyl-Lewis A (Neu5Gc-). Yellow: Positive control. Blue: Negative control.](http://images.novusbio.com/fullsize/CA19-9-Sialyl-Lewis-A-Antibody-GT933-Microarray-NBP3-13650-img0007.jpg "Microarray: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - CA19-9/Sialyl Lewis A antibody [GT933] specifically detects sialyl-Lewis A (Neu5Ac-; CA19-9/Sialyl Lewis A) on a glycan array. Green: sialyl-Lewis A (Neu5Ac-; CA19-9/Sialyl Lewis A). Red: Modified sialyl-Lewis A (Neu5Gc-). Yellow: Positive control. Blue: Negative control.")
![Western Blot: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13650_mouse-ca19-9-sialyl-lewis-a-mab-gt933-91020242091469.jpg "Western Blot: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")