CA19-9/Sialyl Lewis A Antibody (1031516) [Unconjugated]

Capan-2 human pancreatic adenocarcinoma cell line was stained with Mouse Anti-Human CA19-9 Monoclonal Antibody (Catalog # MAB10625, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

CA19-9 was detected in immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line using Mouse Anti-Human CA19-9 Monoclonal Antibody (Catalog # MAB10625) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: 1031516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CA19-9/Sialyl Lewis A Antibody (1031516) [Unconjugated] Summary

• Immunogen: Human CA19-9 from Capan-2 human pancreatic adenocarcinoma cell line
• Specificity: Detects Human CA19-9 in ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CA19-9/Sialyl Lewis A Antibody (1031516) [Unconjugated]

• CA 19-9
• CA19-9

Background

CA19-9 (sialylated Lewis a antigen) is a serum tumor marker for pancreatic cancer diagnosis.  It is also used for monitoring therapy in patients with pancreatic adenocarcinoma.  CA19-9 is synthesized by normal pancreatic and biliary ductal cells and by gastric, colon, endometrial and saliva epithelia.  In a normal patient, it is present in small amounts in serum.  The plasmatic levels of CA19-9 are greatly increased in neoplastic disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.