Bu1 Antibody (AV20)

Images

 
Flow Cytometry: Bu1 Antibody (AV20) [NBP1-28340] - Analysis using the FITC conjugate of NBP1-28340. Staining of 1 ug with CD3-PE on Chicken PBMC.
Flow Cytometry: Bu1 Antibody (AV20) [NBP1-28340] - Analysis using the Biotin conjugate of NBP1-28340. Multiple staining of peripheral blood mononuclear cells.
Flow Cytometry: Bu1 Antibody (AV20) [NBP1-28340] - Analysis using the FITC conjugate of NBP1-28340. Double staining of Chicken peripheral blood mononuclear cells with mouse anti-chicken CD3-R-PE and mouse anti-chicken ...read more

Product Details

Summary
Reactivity ChSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, IP, ICC
Clone
AV20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Catalog# & Conjugate Size Price

Bu1 Antibody (AV20) Summary

Immunogen
Chicken peripheral blood lymphocytes and splenocytes
Specificity
Chicken Bu-1 (chB6), Mr 70-75 kDa
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry

Reactivity Notes

Chicken

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Bu1 Antibody (AV20)

  • B6.1
  • B6.2
  • Bu 1a
  • Bu 1b
  • LOC396098

Background

Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cellassociated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. (1-2) MAb AV20 does not cross react with turkey cells when analyzed by immunofluorescent staining and flow cytometry. (3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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