Bu1 Antibody (AV20) - Azide and BSA Free

Flow Cytometry: Bu1 Antibody (AV20) [NBP1-28340] - Flow Cytometry: Bu1 Antibody (AV20) [FITC] [NBP1-28341] - 1ug w CD3-PE on Chicken PBMC Image using the FITC form of this antibody.

Flow Cytometry: Bu1 Antibody (AV20) [NBP1-28340] - Analysis using the Biotin conjugate of NBP1-28340. Multiple staining of peripheral blood mononuclear cells.

Flow Cytometry: Bu1 Antibody (AV20) [NBP1-28340] - Analysis using the FITC conjugate of NBP1-28340. Double staining of Chicken peripheral blood mononuclear cells with mouse anti-chicken CD3-R-PE and mouse anti-chicken Bu-1-FITC. Lymphocytes were then gated and the cells analyzed on a flow cytometer.

• Reactivity: Ch
• Applications: Flow, IHC, IP, ICC/IF
• Clone: AV20
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Bu1 Antibody (AV20) - Azide and BSA Free Summary

• Immunogen: Chicken peripheral blood lymphocytes and splenocytes
• Specificity: Chicken Bu-1 (chB6), Mr 70-75 kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunocytochemistry
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500

Reactivity Notes

Chicken

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Bu1 Antibody (AV20) - Azide and BSA Free

• B6.1
• B6.2
• Bu 1a
• Bu 1b
• LOC396098

Background

Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cellassociated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. (1-2) MAb AV20 does not cross react with turkey cells when analyzed by immunofluorescent staining and flow cytometry. (3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.