Bu-1b Antibody (5-11G2) [FITC]

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Flow Cytometry: Bu-1b Antibody (5-11G2) [FITC] [NBP1-28324] - Chicken peripheral blood mononuclear cells were first incubated with purified mouse anti-chicken Bu-1b and then counter-stained with goat anti-mouse ...read more

Product Details

Summary
Reactivity ChSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP, ICC
Clone
5-11G2
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

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Catalog# & Conjugate Size Price

Bu-1b Antibody (5-11G2) [FITC] Summary

Immunogen
Bursa cells from two- to five-week-old CHA (Bu-1b) strain chickens
Specificity
Chicken Bu-1b alloantigen, Mr 70 kDa
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Immunocytochemistry

Reactivity Notes

Chicken

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for Bu-1b Antibody (5-11G2) [FITC]

  • B6.1
  • B6.2
  • Bu 1b
  • LOC396098

Background

Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa. (1-2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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