Bu-1a Antibody (21-1A4) - Azide and BSA Free

Flow Cytometry: Bu-1a Antibody (21-1A4) [NBP1-28319] - Analysis using the FITC conjugate of NBP1-28319. Chicken peripheral blood mononuclear cells were first incubated with purified mouse anti-chicken Bu-1a and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using light scatter criteria and analyzed on a flow cytometer.

Flow Cytometry: Bu-1a Antibody (21-1A4) [NBP1-28319] - Analysis using the Biotin conjugate of NBP1-28319. Multiple staining of peripheral blood mononuclear cells.

• Reactivity: Ch
• Applications: Flow
• Clone: 21-1A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Bu-1a Antibody (21-1A4) - Azide and BSA Free Summary

• Immunogen: Bursa cells from one-day-old H.B15 (Bu-1a/b) strain chickens
• Specificity: Chicken Bu-1a/b alloantigen, Mr 70 kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 1 ug/10^6 cells

Reactivity Notes

Chicken

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Bu-1a Antibody (21-1A4) - Azide and BSA Free

• B6.1

Background

Chicken Bu-1a/b, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 21-1A4 does not react with cells from CHA and H.B15 strains by immunofluorescence. (1-2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.