Bromodeoxyuridine/BrdU Antibody (Bu20a)

Product Discontinued

Bromodeoxyuridine/BrdU Antibody (Bu20a) Summary

• Immunogen: Bromodeoxyuridine conjugated to BSA.
• Localization: Nuclear
• Marker: Proliferation Marker
• Specificity: NB100-64345 recognizes the thymidine analogue bromodoxyuridine (BrdU), which can be incorporated into DNA during S-phase of the cell cycle. The BU20a antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labelling techniques.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: For IHC-Frozen: Frozen sections and cell preparations: Acetone-fixed, frozen sections and cell preparations are suitable for staining with clone Bu20a. To survey brain structure overall, use AChE histochemistry, a standard way to identify brain subdivisions. IHC- Formalin-fixed paraffin-embedded tissue sections: Clone Bu20a can be used for labeling paraffin-embedded tissue sections fixed in formalin. Denaturation of the DNA is critical for successful staining of BrdU. This can be achieved by exposing cells to heat, or acid. For heat-induced epitope retrieval, 10mM citrate buffer pH6.0 is recommended. Alternatively, a 30 min incubation in 2M HCl can be performed. The HCl must then be neutralized for 2 min with 0.1 M Na2B4O7. Pretreatment of tissues with proteinase K should be avoided. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Reactivity Notes

Reacts with Chemical

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Bromodeoxyuridine/BrdU Antibody (Bu20a)

• 5-bromo-2-deoxyuridine
• BrdU
• Bromodeoxyuridine
• BUdr

Background

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bromodeoxyuridine/BrdU Antibody (NB100-64345)(3)

Publications using NB100-64345

• Innis, SM et al. Perinatal Lipid Nutrition Alters Early Intestinal Development and Programs the Response to Experimental Colitis in Young Adult Rats. Am J Physiol Gastrointest Liver Physiol. 2010-09-23 [PMID: 20864654]
• Caronia, G et al. Bone morphogenetic protein signaling in the developing telencephalon controls formation of the hippocampal dentate gyrus and modifies fear-related behavior. J Neurosci 30: 6291-301. 2010-01-01 [PMID: 20445055] (IF/IHC)
• Magaud JP, Sargent I, Clarke PJ et al. Double Immunocytochemical labelling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem 37:1517 - 1527. 1989-01-01 [PMID: 2476478]

FAQs for Bromodeoxyuridine/BrdU Antibody (NB100-64345). (Showing 1 - 1 of 1 FAQs).

1. I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
   • We recommend this product: NB500-235​