Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) [HRP]

Product Discontinued

Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) [HRP] Summary

• Immunogen: Made to Chemical BrdU
• Localization: Nuclear
• Marker: Proliferation Marker
• Specificity: This reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts weakly with chlorodeoxyuridine, but does not cross-react with thymidine or iododeoxyuridine.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:25-1:200
  • Immunocytochemistry/ Immunofluorescence 1:200-1:500
  • Immunohistochemistry 1:25-1:200
  • Immunohistochemistry-Paraffin 1:25-1:200
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Monkey reactivity reported in (PMID: 28566378). Rat reactivity reported in (PMID:23966683). Drosophila reactivity reported in (PMID:22438819).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.01% Thimerosal
• Purity: Protein G purified

Alternate Names for Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) [HRP]

• 5-bromo-2-deoxyuridine
• BrdU
• Bromodeoxyuridine
• BUdr

Background

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Bromodeoxyuridine/BrdU Antibody (NB500-169H)(1)

Publication using NB500-169H

• Vanderlaan M, Thomas CB. Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry. 1985-11-01 [PMID: 4064836]

FAQs for Bromodeoxyuridine/BrdU Antibody (NB500-169H). (Showing 1 - 1 of 1 FAQ).

1. I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
   • We recommend this product: NB500-235​