Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) [Alexa Fluor (R) 405]


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Product Details

Reactivity Mu, NASpecies Glossary
Applications Flow, ICC/IF, IHC-Fr, IHC-P
BU1/75 (ICR1)
Please see the vial label for concentration. If unlisted please contact technical services.

Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) [Alexa Fluor (R) 405] Summary

Made to Chemical BrdU
Proliferation Marker
This reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts weakly with chlorodeoxyuridine, but does not cross react with thymidine or iododeoxyuridine.
Protein G purified
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Packaging, Storage & Formulations

Store at 4C in the dark.
Alexa Fluor (R) 405
Alexa Fluor (R) 405
50mM Sodium Borate
0.05% Sodium Azide
Protein G purified


  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Clone BU1/75 (ICR1) can be used for labeling paraffin-embedded tissue sections fixed in formalin. Denaturation of the DNA is critical for successful staining of BrdU. This can be achieved by exposing cells to heat, or acid. For heat-induced epitope retrieval, 10mM citrate buffer pH6.0 is recommended. Alternatively, a 30 min incubation in 2M HCl can be performed. The HCl must then be neutralized for 2 min with 0.1 M Na2B4O7. Pretreatment of tissues with proteinase K should be avoided. Immunohistochemistry-Frozen was reported in scientific literature.

Reactivity Notes


Alternate Names for Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) [Alexa Fluor (R) 405]

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Bromodeoxyuridine/BrdU Antibody (NB500-169AF405) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Additional Bromodeoxyuridine/BrdU Products

Bromodeoxyuridine/BrdU NB500-169AF405

Blogs on Bromodeoxyuridine/BrdU.

BrDU (Bromodeoxyuridine)
The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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