| Reactivity | Ba-CbSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Alexa Fluor 750 |
| Immunogen | E. coli-derived recombinant C. botulinum BoNT-E Light Chain Pro2-Ile403 Accession # P30995 |
| Specificity | Detects C. botulinum BoNT-E Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with rBoNT-B LC is observed and less than 1% cross-reactivity with rBoNT-C HCR and rBoNT-D HCR |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied |
| Buffer | Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide |
Botulinum Neurotoxin Type E is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2‑4). E. coli-expressed recombinant light chains are active proteases. In the absence of the heavy chains, however, they lack toxicity because they cannot enter into host cells.
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
