BoNT-E Heavy Chain Antibody (785208) [Unconjugated]

Western blot shows lysates of recombinantC. botulinumBoNT-E Heavy Chain Receptor Binding Domain (25 ng/lane). PVDF membrane was probed with 1 µg/mL of Mouse Anti-C. botulinumBoNT-E Heavy Chain Monoclonal Antibody (Catalog # MAB7135) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BoNT-E Heavy Chain at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Ba-Cb
• Applications: WB
• Clone: 785208
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BoNT-E Heavy Chain Antibody (785208) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant C. botulinum BoNT-E Heavy Chain
  Lys845-Lys1251
  Accession # P30995
• Specificity: Detects C. botulinum BoNT-E Heavy Chain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant Heavy Chains from BoNT-A or BoNT-G is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Reactivity Notes

C. botulinum

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BoNT-E Heavy Chain Antibody (785208) [Unconjugated]

• BoNTE Heavy Chain
• BoNT-E Heavy Chain
• Bontoxilysin-E

Background

Botulinum Neurotoxin Type E is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2‑4). E. coli-expressed recombinant light chains are active proteases. In the absence of the heavy chains, however, they lack toxicity because they cannot enter into host cells.

1. Campbell K.D. et al. (1993) J. Clin. Microbiol. 31:2255.
2. Montecucco, C. and Giampietro, S. (1993) Trends. Biochem. Sci. 18:324.
3. Turton, K. et al. (2002) Trends. Biochem. Sci. 27:552.
4. Schiavo, G. et al. (2000) Physiol. Rev. 80:717.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P30995(C. botulinum)