![Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) - Azide and BSA Free [NBP3-14093] - Formalin-fixed, paraffin-embedded human kidney. Endogenous biotin stained with Biotin (Vitamin B7 or Vitamin H) antibody (BTN/36).](http://images.novusbio.com/fullsize/nbp3-14093_mouse-biotin-vitamin-b7-or-vitamin-h-mab-btn-36-azide-bsa-free-24120258193556.jpg "Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/36) - Azide and BSA Free [NBP3-14093] - Formalin-fixed, paraffin-embedded human kidney. Endogenous biotin stained with Biotin (Vitamin B7 or Vitamin H) antibody (BTN/36).")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, Flow, Func, ICC/IF, IHC |
| Clone | BTN/36 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13907). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Biotinylated sheep immunoglobulin |
| Localization | Depends on the target |
| Specificity | It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Confocal Microscopy Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes In situ Nucleic Acid Hybridization |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")