Rat beta Galactosidase ELISA Kit (Chemiluminescence) Summary
| Description |
Assay Length: 3 h 30 min |
| Specificity |
This kit recognizes Rat beta Galactosidase in samples. No significant cross-reactivity or interference between Rat beta Galactosidase and analogues was observed |
| Standard Curve Range |
31.25 - 2000 pg/mL |
| Sensitivity |
18.75 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 10 |
| Intra-Assay |
CV% < 10 |
| Spike Recovery |
80 - 120% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Chemiluminescence) |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Biotinylated Detection Ab Diluent
- Certificate of Analysis
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate(100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate (Dismountable)
- Plate Sealer
- Product Description
- Reference Standard & Sample Diluent
- Reference Standard
- Substrate Reagent A
- Substrate Reagent B
|
Alternate Names for Rat beta Galactosidase ELISA Kit (Chemiluminescence)
Background
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for beta Galactosidase ELISA Kit (NBP3-49751). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
Additional beta Galactosidase Products
Blogs on beta Galactosidase
![Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).](https://images.novusbio.com/images/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0014.jpg "Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).")