Basal cell cytokeratin Antibody (RCK103) - BSA Free

Immunocytochemistry/Immunofluorescence: Basal cell cytokeratin Antibody (RCK103) [NBP1-97729] - Staining of a 7 days old zebrafish embryo, showing positive reactivity in epithelia lining the intestine and internal organs

Immunohistochemistry-Frozen: Basal cell cytokeratin Antibody (RCK103) [NBP1-97729] - Frozen sections of human skin

• Reactivity: Hu, Rt, Po, Av, Ca, Ch, Gp, Ha, Rb, Ze
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: RCK103
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Basal cell cytokeratin Antibody (RCK103) - BSA Free Summary

• Description: The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
• Immunogen: Derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a mix of cell preparations containing Human Cytokeratins.
• Specificity: This antibody is a Cytokeratin antibody recognizing (amongst others) Cytokeratin 5. This monoclonal antibody stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100-1:200
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100-1:200
  • Western Blot 1:100-1:1000
• Application Notes: Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
  
  This antibody is CyTOF ready..

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.