Atrazine Antibody

• Reactivity: All-NA
• Applications: ELISA, AP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

Atrazine Antibody Summary

• Description: Store at 4C or aliquot and store at -20 C for longer periods. Precautions should be taken upon storage for longer periods. Problems of long term stability may occur with highly diluted solutions. For long-term storage purposes in solution the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.
• Immunogen: Serum of sheep immunised with BSA-atrazine conjugate, and purified using caprylic acid
• Specificity: Immunogen: BSA-atrazine conjugate, Target: Atrazine, CAS no.: 1912-24-9. The specificity of the antibody was determined by measuring the cross-reactivity with a range of compounds in ELISA.
  
  Cross Reactivity:
  
  Atrazin: 100%
  
  Propazine: 15%
  
  Simazine: 4%
  
  Ametryn: 2500%
  
  Prometryn: 1700%
  
  Simetryn: 280%
  
  Terbutylazine: 2%
  
  Atrazine-desethyl: 7%
  
  Atrazine desisopropyl 1%
  
  Atrazine-2-hydroxy: 1%
  
  Cinosulfuron: <0.1%
  
  Prosulferon <0.1%
  
  Triasulfuron: <0.1%
  
  Chlorpyrifos: <0.1%Clofibrinezuur <0.1%
  
  Deltametrin <0.1%
  
  Diclofenac: 1%
  
  Etrimfos: 1%
  
  Erythromycine <0.1%
  
  Fenitrothion: <0.1%
  
  Fenofibraat <0.1%
  
  Atrazine: 21%
  
  Ibuprofen: <0.1%
  
  Malathion: <0.1%
  
  Maleaminezuur: <0.1%
  
  Metoprolol: <0.1%
  
  Metacrifos: <0.1%
  
  4-n-Nonylfenol: 2%
  
  Permetrin: <0.1%
  
  Pirimifos-ethyl: <0.1%
  
  Sulfadimidine: <0.1%
  
  Vinclozolin: <0.1%
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity: Caprylic acid precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:500
  • Immunoaffinity Purification
• Application Notes: Depending on conjugate: dilution 1:500 . (The titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Suggested concentration to use in ELISA: 1:500 from the delivered solution. Plates are coated with 400 ng/ml OVA-conjugated Atrazine. HRP-conjugated anti-sheep IgG as a tracer 1:8,000
  
  Immuno-affinity-chromatography.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: 0.02% Sodium Azide
• Purity: Caprylic acid precipitation

Alternate Names for Atrazine Antibody

• 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine

Background

Atrazine is a herbicide against pre- and post-emergence broadleaf and grassy weeds. It is a very persistent compound and it has been banned in the EU since 2004. Its main toxic effect is as endocrine diruptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.