Astrocytomas Antibody (J1-31)

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
J1-31
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Astrocytomas Antibody (J1-31) Summary

Immunogen
Human cerebral white matter plaque materials from a multiple sclerosis patient.
Specificity
The antibody recognizes an intracellular protein antigen (MW 30 kDa) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS).The antibody has recently been found to be a specific marker for low grade astrocytoma in human brain tissue. The antibody is able to distinguish between low grade astrocytoma and normal reactive gliosis (patent application filed). Monoclonal antibody J1-31 was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP {Malhotra, SK (1989) J Neurosci Res. 22(1):36-49}.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
Unpurified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Western Blot: reducing conditions, recognizes a 30kDa protein {Singh, R et al (1986) Biosci Rep 6(1):73-80. Immunohistochemistry The antibody works on paraffin embedded tissue sections. Immunocytochemistry/Immunofluorescence

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
No Preservative
Purity
Unpurified

Alternate Names for Astrocytomas Antibody (J1-31)

  • Astrocytoma Marker
  • Astrocytomas Marker
  • Low Grade Astrocytoma Marker

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Additional Astrocytomas Products

Array NBP2-29820

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