ANKRD53 Antibody (OTI1E1) - Azide and BSA Free

Images

 
Western Blot: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANKRD53 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ANKRD53.
Immunohistochemistry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - Staining of paraffin-embedded Human lymph node tissue using anti-ANKRD53 mouse monoclonal antibody.
Flow Cytometry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ANKRD53 ...read more
Western Blot: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ANKRD53 monoclonal antibody.
Immunohistochemistry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - Staining of paraffin-embedded Human lymphoma tissue using anti-ANKRD53 mouse monoclonal antibody.
Flow Cytometry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - Analysis of Hela cells, using anti-ANKRD53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: ANKRD53 Antibody (OTI1E1) - Azide and BSA Free [NBP2-72217] - Analysis of Jurkat cells, using anti-ANKRD53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

ANKRD53 Antibody (OTI1E1) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-300 of human ANKRD53 (NP_079209) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ANKRD53
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • CyTOF-ready
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for ANKRD53 Antibody (OTI1E1) - Azide and BSA Free

  • ankyrin repeat domain 53
  • ankyrin repeat domain-containing protein 53
  • FLJ12056
  • FLJ36160

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ANKRD53 Antibody (NBP2-72217) (0)

There are no publications for ANKRD53 Antibody (NBP2-72217).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ANKRD53 Antibody (NBP2-72217) (0)

There are no reviews for ANKRD53 Antibody (NBP2-72217). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ANKRD53 Antibody (NBP2-72217) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-72217AF350
Alexa Fluor 405 NBP2-72217AF405
Alexa Fluor 488 NBP2-72217AF488
Alexa Fluor 532 NBP2-72217AF532
Alexa Fluor 594 NBP2-72217AF594
Alexa Fluor 647 NBP2-72217AF647
Alexa Fluor 700 NBP2-72217AF700
Alexa Fluor 750 NBP2-72217AF750
Allophycocyanin NBP2-72217APC
Allophycocyanin/Cy7 NBP2-72217APCCY7
Biotin NBP2-72217B
DyLight 350 NBP2-72217UV
DyLight 405 NBP2-72217V
DyLight 488 NBP2-72217G
DyLight 550 NBP2-72217R
DyLight 594 NBP2-72217DL594
DyLight 650 NBP2-72217C
DyLight 680 NBP2-72217FR
DyLight 755 NBP2-72217IR
FITC NBP2-72217F
HRP NBP2-72217H
Janelia Fluor 549 NBP2-72217JF549
Janelia Fluor 646 NBP2-72217JF646
PE NBP2-72217PE
PE/Atto594 NBP2-72217PEATT594
PE/Cy5.5 NBP2-72217PECY55
PE/Cy7 NBP2-72217PECY7
PerCP NBP2-72217PCP

Additional ANKRD53 Products

Bioinformatics Tool for ANKRD53 Antibody (NBP2-72217)

Discover related pathways, diseases and genes to ANKRD53 Antibody (NBP2-72217). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on ANKRD53

There are no specific blogs for ANKRD53, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ANKRD53 Antibody (OTI1E1) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol ANKRD53