Aggressive Tumor Markers Antibody Pack

Western Blot: Aggressive Tumor Markers Antibody Pack [NBP1-78939] - Detection of p19ARF in MEF lysate (25ug) (2ug/ml) using NB200-106

Immunohistochemistry: Aggressive Tumor Markers Antibody Pack [NBP1-78939] - Analysis of FFPE mouse spleen using p16INK4a/CDKN2A antibody (NB200-106)at 10 ug/mL on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.

Western Blot: Aggressive Tumor Markers Antibody Pack 2vials [NBP1-78939] - Detection of Cathepsin E in 10 ug of murine reticulocyte lysate using NB400-152.

Immunohistochemistry: Aggressive Tumor Markers Antibody Pack [NBP1-78939] - Analysis of Cathepsin E with NB400-152 in human renal cancer using DAB and hematoxylin counterstain.

Product Discontinued

Aggressive Tumor Markers Antibody Pack Summary

• Description: This pack contains 1 vial each of: NB400-152 (25 uL) and NB200-106 (25 uL).
• Immunogen: See individual datasheets.
• Specificity: This Aggressive Tumor Marker Antibodies Sample Pack includes antibodies to p19ARF and Cathepsin E.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Mainly immunofluorescence and Western blot. However, additional applications may be possible for individual products.
  See individual datasheets for validated applications.

Reactivity Notes

Mouse, however additional species may be possible for individual products.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: See individual datasheets.

Background

Tumor grade refers to a measure of how abnormal cells from your tumor appear under the microscope. This can refer to the appearance of the cells or to the percentage that appear to be dividing. The higher the grade, the more aggressive and fast growing the cancer. Tumors are typically classified from least to most aggressive as grade I through IV. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. Cathepsin E (CTSE) is an intracellular aspartic protease belonging to pepsin superfamily and is highly homologous to the analogous aspartic protease cathepsin D. Human and mice subjects with a shortage of CTSE are often prone to atopic dermatitis. Increased levels of CTSE are observed in proliferative diseases such as gastric carcinoma, cervical cancer, and has been considered a promising marker for pancreatic duct cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.