Recombinant Aeromonas Aminopeptidase Protein

SDS-Page: Recombinant Bacterial Aeromonas Aminopeptidase Protein [NBP2-34873]

• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Aeromonas Aminopeptidase Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 291 amino acids corresponding to Aeromonas Aminopeptidase Source: E. coli

  Uniprot ID: Q01693

  Amino Acid Sequence: MPPITQQATV TAWLPQVDAS QITGTISSLE SFTNRFYTTT SGAQASDWIA SEWQALSASL PNASVKQVSH SGYNQKSVVM TITGSEAPDE WIVIGGHLDS TIGSHTNEQS VAPGADDDAS GIAAVTEVIR VLSENNFQPK RSIAFMAYAA EEVGLRGSQD LANQYKSEGK NVVSALQLDM TNYKGSAQDV VFITDYTDSN FTQYLTQLMD EYLPSLTYGF DTCGYACSDH ASWHNAGYPA AMPFESKFND YNPRIHTTQD TLANSDPTGS HAKKFTQLGL AYAIEMGSAT G

• Details of Functionality: Bacterial leucyl aminopeptisdase protein sequentially cleaves N-terminal amino acids except E, D, and X-P.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of Aeromonas Aminopeptidase as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 31.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 150 mM NaCl, 5uM ZnSO4, pH 8.0.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Aeromonas Aminopeptidase Protein

• Aeromonas Aminopeptidase
• Aminopeptidase
• Bacterial leucyl aminopeptidase, EC 3.4.11.10.
• Vibrio proteolyticus (Aeromonas proteolytica)

Background

Aminopeptidases catalyze the cleavage of amino acids from the amino terminus of protein or peptide substrates. They are widely distributed throughout the animal and plant kingdoms and are found in many subcellular organelles, in cytoplasm and as membrane components. Some aminopeptidases are monomeric and others are assemblies of relatively high mass (50 kDa) subunits. Aminopeptidases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.