Acrolein Antibody

Product Discontinued

Acrolein Antibody Summary

• Immunogen: Synthetic acrolein conjugated to protein carrier (PC).
• Specificity: Recognizes conjugated Acrolein. Using a conjugate Acrolein Protein, specificity was performed with an ELISA test by competition experiments with the following compounds. Acrolein-BSA (ratio 1:1), Malondialdehyde-BSA (1:55), Glyoxal-BSA (1:>50,000), Formaldehyde-BSA (1:>50,000), Glutaraldehyde-BSA (1:> 50,000)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 1:1000-1:2000
• Application Notes: Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25505195). Use in Dot Blot reported in scientific literature ( PMID 25446876)

Reactivity Notes

Recognizes conjugated Acrolein.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized deionized water.

Alternate Names for Acrolein Antibody

• Acrylic amide; Ethylene Carboxamide 2-Propenamide; 2-Propen-1-one; 2-propenal; Acraldehyde; Acrolein; Acrylic aldehyde

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Acrolein Antibody (NB200-556)(11)

Publications using NB200-556

• Phan K, He Y, Pickford R et Al. Uncovering pathophysiological changes in frontotemporal dementia using serum lipids Sci Rep 2020-02-27 [PMID: 32107421] (WB, Human)
• Baba SP, Zhang D, Singh M et al. Deficiency of aldose reductase exacerbates early pressure overload-induced cardiac dysfunction and autophagy in mice. J. Mol. Cell. Cardiol. 2018-04-05 [PMID: 29627295] (Mouse)
• Hall SE, Aitken RJ, Nixon B et al. Electrophilic aldehyde products of lipid peroxidation selectively adduct to heat shock protein 90 and arylsulfatase A in stallion spermatozoa. Biology of Reproduction. 2017-01-17 [PMID: 28395341]
• Marquis A, Packer RA, Borgens RB, Duerstock BS. Increase in oxidative stress biomarkers in dogs with ascending-descending myelomalacia following spinal cord injury J. Neurol. Sci. 2015-04-14 [PMID: 25912174] (WB, Canine)
• Lord T, Martin JH, Aitken RJ. Accumulation of Electrophilic Aldehydes During Post-Ovulatory Ageing of Mouse Oocytes Causes Reduced Fertility, Oxidative Stress, and Apoptosis. Biol. Reprod. 2014-12-10 [PMID: 25505195] (ICC/IF, WB, Mouse)
• Tully M, Zheng L, Acosta G et al. Acute systemic accumulation of acrolein in mice by inhalation at a concentration similar to that in cigarette smoke. Neurosci Bull. 2014-12-01 [PMID: 25446876] (Cytometric Bead Assay Standard, Mouse)
  
  Details:
  Acrolein antibody used in Dot Blot application for detecting the accumulation of acrolein-lysine adducts in the spinal cords of mice following nasal exposure to acrolein. Fig 3 shows the representative dot blots for control, acrolein, and sham groups.
• Kitaguchi Y, Taraseviciene-Stewart L, Hanaoka M, Natarajan R, Kraskauskas D et al. Acrolein Induces Endoplasmic Reticulum Stress and Causes Airspace Enlargement. PLoS ONE 7(5): e38038. 2012-01-01 [PMID: 22675432] (IF/IHC, Human)
• Bruban J, Maoui A, Chalour N et al. CCR2/CCL2-mediated inflammation protects photoreceptor cells from amyloid-beta-induced apoptosis. Neurobiol Dis;42(1):55-72. 2011-04-01 [PMID: 21220018]
• Leung G, Sun W, Zheng L et al. Anti-acrolein treatment improves behavioral outcome and alleviates myelin damage in experimental autoimmune encephalomyelitis mouse Neuroscience 2011-01-01 [PMID: 21081153] (WB)
• Mizukami S, Ichimura R, Kemmochi S et al. Induction of GST-P-positive proliferative lesions facilitating lipid peroxidation with possible involvement of transferrin receptor up-regulation and ceruloplasmin down-regulation from the early stage of liver tumor promotion in rats. Arch Toxicol 2010-04-01 [PMID: 20091025] (IHC-P)
• Hammnan et al. CRITICAL ROLE OF ACROLEIN IN SECONDARY INJURY FOLLOWING EX VIVO SPINAL CORD TRAUMA. J Neurochem; 107(3): 712-721. 2008-11-01 [PMID: 18710419] (WB, IF/IHC)

FAQs for Acrolein Antibody (NB200-556). (Showing 1 - 2 of 2 FAQs).

1. Could you please let us know the Molecular Weight of NB200-556 for WB?
   • Acrolein is a small organic compound (it is an unsaturated aldehyde; not a protein). NB200-556 recognizes acrolein-modifed proteins; the molecular weight will depend on the MW of the protein to which the acrolein is conjugated to (protein modification by acrolein can be ascribed to the formation of acrolein-lysine and/or acrolein-histidine adducts that possess a carbonyl function). This also applies to the size of the protein.
2. I have recently purchased Acrolein antibody (NB200-556) and used for western blotting. I have got two bands one is very faint (50Kda) and second nice band at 5-15 Kda. I was wondering at what location band for Acrolein (mol wt.) should show up in western blotting. Should I consider faint band (50KDa) also ??
   • Acrolein is a simple aldehyde. Detecting this molecule alone is impossible on Western blot as it is far too small. If it is contained in your protein or molecule of interest, it will be detected at the molecular weight of the protein or molecule. Because of this, it is impossible to give you a size to look for as everyone's samples will be different. Since this is a very unique antibody, I would treat any band that shows up when detecting with NB200-556 as being Acrolein positive.