Acrolein Antibodies
We offer Acrolein Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each Acrolein Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Acrolein Antibodies can be used in a variety of model species. Use the list below to choose the Acrolein Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Acrolein polyclonal antibodies.
Alternate Names for Acrolein Antibodies
anti-Acrolein antibody, anti-Acrylic amide; Ethylene Carboxamide 2-Propenamide; 2-Propen-1-one; 2-propenal; Acraldehyde; Acrolein; Acrylic aldehyde antibody
2 Results for "acrolein" in Primary Antibodies
![Western Blot: Acrolein Antibody (10A10) [NBP2-59359] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Western-Blot-NBP2-59359-img0012.jpg "Western Blot: Acrolein Antibody (10A10) [NBP2-59359] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.")
![Immunocytochemistry/Immunofluorescence: Acrolein Antibody (10A10) [NBP2-59359] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Immunocytochemistry-Immunofluorescence-NBP2-59359-img0002.jpg)
![Immunohistochemistry: Acrolein Antibody (10A10) [NBP2-59359] - Immunohistochemistry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Adrenal Carcinoma. Species: Human. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Immunohistochemistry-NBP2-59359-img0010.jpg)
![Flow Cytometry: Acrolein Antibody (10A10) [NBP2-59359] - Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Flow-Cytometry-NBP2-59359-img0013.jpg)
![Western Blot: Acrolein Antibody (10A10) [NBP2-59359] - Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10 (NBP2-59359). Lane 1: Molecular Weight Ladder (MW). Lane 2: AcroleinBSA (0.5 ug). Lane 3: AcroleinBSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59359) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/Acrolein-Antibody-10A10-Western-Blot-NBP2-59359-img0011.jpg)
![Western Blot: Acrolein Antibody (2H2) [NBP2-59358] - Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (NBP2-59358). Lane 1: Molecular Weight Ladder (MW). Lane 2: Acrolein-BSA (0.5 ug). Lane 3: Acrolein-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59358) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/Acrolein-Antibody-2H2-Western-Blot-NBP2-59358-img0007.jpg "Western Blot: Acrolein Antibody (2H2) [NBP2-59358] - Western Blot analysis of Acrolein-BSA Conjugate showing detection of 67 kDa Acrolein protein using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (NBP2-59358). Lane 1: Molecular Weight Ladder (MW). Lane 2: Acrolein-BSA (0.5 ug). Lane 3: Acrolein-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59358) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.")
![Immunocytochemistry/Immunofluorescence: Acrolein Antibody (2H2) [NBP2-59358] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (NBP2-59358). Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59358) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 M H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Acrolein-Antibody-2H2-Immunocytochemistry-Immunofluorescence-NBP2-59358-img0008.jpg)
![Flow Cytometry: Acrolein Antibody (2H2) [NBP2-59358] - Flow Cytometry analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2 (NBP2-59358). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody (NBP2-59358) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.](http://images.novusbio.com/fullsize/Acrolein-Antibody-2H2-Flow-Cytometry-NBP2-59358-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Acrolein Antibody (2H2) [NBP2-59358] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Acrolein-Antibody-2H2-Immunocytochemistry-Immunofluorescence-NBP2-59358-img0002.jpg)