8-OHdG Antibody

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Immunohistochemistry: 8-Hydroxyguanosine Antibody [NB600-1508] - Goat anti-8-OHdG Alzheimer disease brain showing immunohistochemcal staining of oxidized DNA in neurons. Tissue sections were formalin-fixed, ...read more
Immunohistochemistry: 8-Hydroxyguanosine Antibody [NB600-1508] - Goat anti-8-OHdG Alzheimer disease brain showing immunohistochemical staining of oxidized DNA in neurons. Tissue sections were formalin-fixed, ...read more
ELISA: 8-OHdG Antibody [NB600-1508] - Competitive ELISA using the 8-OHG antibody demonstrates a high specificity towards 8-hydroxydeoxyguanosine (8-OHdG) and 8-hydroxyguanosine nucleotides. Partial cross-reactivity is ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

8-OHdG Antibody Summary

Immunogen
8-Hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine.
Localization
Nuclear
Specificity
NB600-1508 cross reacts completely with 8-OHdG, and does not cross react with other naturally occurring nucleotides.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100000-1:250000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
For IHC-Paraffin: It is suggested that the tissues are pretreated with 10 ug/mL of proteinase K for 40 minutes at 37 degrees C prior to staining. Dewaxed sections treated with 3%H2O2 in methanol for 30 minutes and blocking was with 10% serum in TBS. Sections were treated with proteinase K 10ug/ml in PBS pH 7.4, 40 minutes 37 degrees C prior to blocking. Detection is via DAB. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier. Use in ICC/IF reported in scientific literature (PMID: 28269997).
Publications
Read Publications using
NB600-1508 in the following applications:

Reactivity Notes

Due to the conserved nature of the target cross reactivity would be expected in wide range of species. Mouse reactivity reported in scientific literature (PMID: 29559852).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.01% Thimerosal
Purity
Unpurified

Alternate Names for 8-OHdG Antibody

  • 8 OHG
  • 8-hydroxy-2
  • 8-hydroxydeoxyguanosine
  • 8-Hydroxyguanine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for 8-OHdG Antibody (NB600-1508)(3)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for 8-OHdG Antibody (NB600-1508). (Showing 1 - 1 of 1 FAQs).

  1. NB600-1508 could be applied on mouse tissue?
    • Since 8-hydroxyguanosine is a modified base which occurs in DNA as a result of oxidative stress, hence considered a sensitive marker of oxidative damage in cellular DNA, its structure is pretty conserved among different species. Therefore, the cross reactivity of this antibody may be expected in wide range of species. It is worth mentioning NB600-1508 does not cross react with other naturally occurring nucleotides.

Secondary Antibodies

 

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