![Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-59182] - An hydroxymethylated DNA IP (hMeDIP) was performed using the rabbit polyclonal antibody directed against 5-hydroxymethylcytosine. The IgG isotype antibodies from rabbit were used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated to have DNA fragments of 300-500 bp. 1 ug of human HeLa cell DNA was spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP'd material was analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the rabbit polyclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments).](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-Methylated-DNA-Immunoprecipitation-NBP2-59182-img0002.jpg "Methylated DNA Immunoprecipitation: 5-hmC Antibody [NBP2-59182] - An hydroxymethylated DNA IP (hMeDIP) was performed using the rabbit polyclonal antibody directed against 5-hydroxymethylcytosine. The IgG isotype antibodies from rabbit were used as negative control. The DNA was prepared with the GenDNA module of the hMeDIP kit and sonicated to have DNA fragments of 300-500 bp. 1 ug of human HeLa cell DNA was spiked with non-methylated, methylated, and hydroxymethylated fragments. The IP'd material was analysed by qPCR using the primer pair specific for the 3 different control sequences. The obtained results show that the rabbit polyclonal for 5-hmC is highly specific for this base modification (no IP with non-methylated or methylated C bases containing fragments).")
| Immunogen | The exact immunogen is propietary information. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Serum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
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![ELISA: 5-hmC Antibody [NBP2-59182] - To determine the titer, an ELISA was performed using a serial dilution of the rabbit polyclonal antibody directed against 5-hmC in antigen coated wells. The antigen used was BSA coupled to the 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 3,500.](http://images.novusbio.com/fullsize/5-Hydroxymethylcytosine-Antibody-ELISA-NBP2-59182-img0001.jpg "ELISA: 5-hmC Antibody [NBP2-59182] - To determine the titer, an ELISA was performed using a serial dilution of the rabbit polyclonal antibody directed against 5-hmC in antigen coated wells. The antigen used was BSA coupled to the 5-hmC base. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1: 3,500.")
![Dot Blot: 5-hmC Antibody [NBP2-59182] - 100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the rabbit 5-hydroxymethylcytosine polyclonal antibody (dilution 1:200). The membranes were exposed for 30 seconds.](http://images.novusbio.com/fullsize/5-hmC-Antibody-Dot-Blot-NBP2-59182-img0005.jpg "Dot Blot: 5-hmC Antibody [NBP2-59182] - 100 to 4 ng (equivalent of 5 to 0.2 pmol of C-bases) of the hmC, mC and C PCR controls were spotted on a membrane (Amersham Hybond-N+). The membrane was incubated with the rabbit 5-hydroxymethylcytosine polyclonal antibody (dilution 1:200). The membranes were exposed for 30 seconds.")