5-Carboxylcytosine Antibody Pair [Biotin]

• Reactivity: Hu
• Applications: ELISA, ELISA(Cap), ELISA(Det)
• Conjugate: Biotin

5-Carboxylcytosine Antibody Pair [Biotin] Summary

• Assay Type: Sandwich ELISA

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 50 - 200ng/well
  • ELISA Detection (Matched Antibody Pair) 0.1 - 0.5ug/ml
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.

Alternate Names for 5-Carboxylcytosine Antibody Pair [Biotin]

• 5-caC

Background

5-carboxylcytosine (5caC) is the final oxidized derivative of 5-methylcytosine (5mC). 5mC is oxidized to 5-hydroxymethylcytosine (5hmC) which is then oxidized to 5-formylcytosine (5fC) then 5caC. Each of these oxidation steps are catalyzed by the Ten-Eleven Translocation (TET) family of enzymes. 5fC can then be further oxidized to 5-carboxylcytosine (5caC) by TET. Both 5fC and 5caC can be converted to unmodified cytosine by Terminal deoxynucleotidyl transferase (TdT) or Thymine DNA glycosylase (TDG) by base excision repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.