Mouse 4-Hydroxynonenal ELISA Kit (Colorimetric)

4-Hydroxynonenal ELISA Kit (Colorimetric) - Standard Curve Reference

Product Discontinued

Mouse 4-Hydroxynonenal ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse 4-HNE in samples. No significant cross-reactivity or interference between Mouse 4-HNE and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 4.77%
• Intra-Assay: CV% < 4.54%
• Spike Recovery: 87-107%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Alternate Names for Mouse 4-Hydroxynonenal ELISA Kit (Colorimetric)

• (2E)-4-Hydroxy-2-nonenal
• 2-Nonenal, 4-hydroxy-, (2E,4R)-
• 4 HNE
• 4Hydroxynonenal
• 4-Hydroxynonenal
• 4-hydroxy-trans-2-nonenal
• HNE

Background

4-HNE is a hydroxyalkenal which is produced by lipid peroxidation in cells. It is found in higher quantities during stress, due to the increase in the lipid peroxidation reaction that accompanies stress events. 4-Hydroxynonenanal is believed to play a key role in signal transduction in a number of pathways, including cellular adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for 4-Hydroxynonenal ELISA Kit (NBP2-66365). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.