4-Hydroxynonenal Antibody

Images

 
Western Blot: 4-Hydroxynonenal Antibody [NB100-63093] - Western blot of aged control and Alzheimer brain homogenates using 1:3,000 dilution of 4-HNE antiserum.

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

4-Hydroxynonenal Antibody Summary

Immunogen
4-hydroxynonenal conjugate
Localization
Cytoplasmic
Marker
Lipid Peroxidation Marker
Specificity
NB100-63093 recognises 4-hydroxynonenal (HNE). This antiserum has been shown to be immunoreactive with 4-HNE-modified bovine serum albumin (BSA) by ELISA. Additionally, this antibody has been shown by ELISA to have minimum cross-reactivity with other aldehyde-BSA conjugates, making it highly specific for 4-HNE and capable of detecting proteins modified by HNE-adducts.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:3000
  • ELISA 1:10000
  • Immunohistochemistry
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 27485360).
Reviewed Applications
Read 1 Review rated 4
using
NB100-63093 in the following applications:

Publications
Read Publications using
NB100-63093 in the following applications:

  • IHC
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for 4-Hydroxynonenal Antibody

  • 4 HNE
  • 4Hydroxynonenal
  • 4-Hydroxynonenal
  • HNE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 4-Hydroxynonenal Antibody (NB100-63093)(8)

We have publications tested in 2 confirmed species: Rat, Mouse .

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(2)
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Rat
(3)
Mouse
(1)
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Showing Publications 1 - 8 of 8.
Publications using NB100-63093 Applications Species
Newberry EP, Xie Y, Kennedy SM et al. Prevention of hepatic fibrosis with liver microsomal triglyceride transfer protein deletion in Liver fatty acid binding protein null mice. Hepatology. Nov 14 2016 [PMID: 27862118]
Carbonell T, Sanchez-Nuno NA, Dewey S, Gomes AV. Subnormothermic perfusion in the isolated rat liver preserves the antioxidant glutathione and enhances the function of the ubiquitin proteasome system Oxid Med Cell Longev Sep 15 2016 [PMID: 27800122] (WB, Rat) WB Rat
Gajbhiye R, Bendigeri T, Ghuge A et al. Panel of Autoimmune Markers for Noninvasive Diagnosis of Minimal-Mild Endometriosis: A Multicenter Study Reprod Sci 2016 Jul 08 [PMID: 27485360] (IHC, Rat) IHC Rat
Barone E, Di Domenico F, Cassano T et al. Impairment of biliverdin reductase-A promotes brain insulin resistance in Alzheimer disease: A new paradigm. Free Radic Biol Med 2016 Feb [PMID: 26698666]
Barone E, Cenini G, Di Domenico F et al. Basal brain oxidative and nitrative stress levels are finely regulated by the interplay between superoxide dismutase 2 and p53. J. Neurosci. Res. 2015 Aug 06 [PMID: 26251011] (Mouse )

Details:
4-Hydroxynonenal antibody was used at 1:2000 dilution for Slot-blot assay on nuclear, cytosolic or mitochondrial fractions and whole homogenates from brains of WT, SOD2 transgenic (TgSOD2), and SOD2 heterozygous knockout (SOD2?/+) mice and of mice carrying the double mutation (p53?/? × TgSOD2, p53?/? × SOD2?/+).
Mouse
Gong D, Zhang Y, Zhang H et al. Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia-Reperfusion Injury Cardiovasc Toxicol 2012 Jul 20 [PMID: 22814936] (WB, Rat) WB Rat
Agarwal R, Hennings L, Rafferty TM et al. Acetaminophen-induced hepatotoxicity and protein nitration in neuronal nitric-oxide synthase knockout mice J Pharmacol Exp Ther 2012 Jan [PMID: 22001257]
Almeida M, Ambrogini E, Han L et al. Increased Lipid Oxidation Causes Oxidative Stress, Increased Peroxisome Proliferator-activated Receptor-{gamma} Expression, and Diminished Pro-osteogenic Wnt Signaling in the Skeleton. J Biol Chem;28440:27438-27448. 2009 [PMID: 19657144]

Review for 4-Hydroxynonenal Antibody (NB100-63093) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB100-63093:
Filter by Applications
WB
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 12/22/2014
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22001257
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22001257

Details

Detection NotesSee PMID 22001257

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22001257.

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Secondary Antibodies

 

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Additional 4-Hydroxynonenal Products

4-Hydroxynonenal NB100-63093

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Recent Reviews

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Anonymous
12/22/2014
Application: WB
Species: Mouse