20S Proteasome Antibody (863437) [Unconjugated]


20S Proteasome was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human 20S Proteasome Monoclonal Antibody (Catalog # MAB9554) at 8 µg/mL for 3 hours at room temperature. Cells ...read more
Western blot shows Human 20S Proteasome (Catalog # E-360), Human 20S Immunoproteasome (Catalog # E-370), and Recombinant Human PSMA1. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human 20S Proteasome ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF

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20S Proteasome Antibody (863437) [Unconjugated] Summary

Human 20S Proteasome purified from human erthrocytes
Detects human 20S Proteasome in direct ELISAs and Western blots.
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  • Western Blot 2 ug/mL
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 20S Proteasome Antibody (863437) [Unconjugated]

  • 20S Proteasome


The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle and is the catalytic subunit of the multi-complex Immunoproteasome. The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 non-identical subunits arranged into four stacked rings. However, during 20S Immunoproteasome assembly, the three catalytic beta subunits, beta 1, 2, and 5, in the two interior rings of the 20S Proteasome are replaced by three IFN-gamma-inducible catalytic subunits: beta 1i/LMP2, beta 2i/LMP7, and beta 5i/MECL-1. The 20S Immunoproteasome is commonly associated with the 19S, PA28 alpha/beta, or the PA28 gamma regulatory complexes. 20S Immunoproteasome expression is enriched in antigen presenting cells of the immune system where the 20S Immunoproteasome selectively degrades intracellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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