ZFYVE21 Antibodies
We offer ZFYVE21 Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each ZFYVE21 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ZFYVE21 Antibodies can be used in a variety of model species: Human. Use the list below to choose the ZFYVE21 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ZFYVE21 polyclonal antibodies and browse our ZFYVE21 monoclonal antibody catalog.
Alternate Names for ZFYVE21 Antibodies
anti-ZFYVE21 antibody, anti-FYVE domain containing 21 antibody, anti-ZF21MGC2550 antibody, anti-zinc finger FYVE domain-containing protein 21 antibody, anti-zinc finger, FYVE domain containing 21 antibody
9 Results for "zfyve21" in Primary Antibodies
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Membrane attack complex-induced and endosome-dependent stabilization of ZFYVE21. Human umbilical vein endothelial cells (HUVECs) were treated with panel reactive antibody (PRA) sera (25% v/v in gelatin veronal buffer) (a). HUVECs were treated with MG132 (25uM). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0005.jpg "Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Membrane attack complex-induced and endosome-dependent stabilization of ZFYVE21. Human umbilical vein endothelial cells (HUVECs) were treated with panel reactive antibody (PRA) sera (25% v/v in gelatin veronal buffer) (a). HUVECs were treated with MG132 (25uM). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.")
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Analysis of ZFYVE21 expression in transfected 293T cell line by ZFYVE21 polyclonal antibody. Lane 1: ZFYVE21 transfected lysate(25.74 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Correlations of ZFYVE21 and complement in patient tissues. Western blots of human umbilical vein endothelial cells treated with either panel reactive antibody (30min, 25% v/v in gelatin veronal buffer) or LIGHT (12h, 50ng/mL in EGM2 media, a). Kidney biopsies from renal transplant patients (n=12) were stained as indicated. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0003.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Recombinant FLAG-tagged ZFYVE21 (1ug) and/or recombinant SMURF2 (1ug) protein was incubated for 30min at room temperature and analyzed by western blot. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-019-10041-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-H00079038-B01P-img0004.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - Correlations of ZFYVE21 & complement in patient tissues. Western blots of human umbilical vein endothelial cells treated with either panel reactive antibody (30 min, 25% v/v in gelatin veronal buffer) or LIGHT (12 h, 50 ng/mL in EGM2 media, a). Kidney biopsies from renal transplant patients (n = 12) were stained as indicated (b). Synovial biopsies from osteoarthritis (n = 11), low-titer rheumatoid factor antibody rheumatoid arthritis (RF RA; n = 8), & high-titer RF RA (n = 8) were stained as indicated (c–e) & spatial correlations between ZFVYE21 or nuclear factor-inducing kinase (NIK) with endothelial cells (Ulex, f) & membrane attack complex (polyC9, g) were assessed using the computer software (scale bar: 200 μm). ****p < 1 × 10−5, Student’s t test. CD45 abundance was assessed (h) & correlated with NIK staining (i) via blinded scoring from three independent observers. *p < 0.05, **p < 0.001. Western blotting was repeated three times. Analysis of variance statistical analyses were performed. Representative data shown *p < 0.05, **p < 0.001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31113953), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00079038-b01p_mouse-polyclonal-zfyve21-antibody-31020241548367.jpg)
![Western Blot: ZFYVE21 Antibody [H00079038-B01P] - SMURF2-dependent degradation of vesicular PTEN. PTEN co-immunoprecipitations (co-IPs) were performed on human umbilical vein endothelial cells (HUVECs) treated for 30 min with panel reactive antibody (PRA) sera & blotted as indicated (a). Rab5 co-IPs were performed on PRA-treated HUVECs transfected with control or SMURF2 siRNA (b). Western blot analysis of whole-cell lysates of HUVECs transiently transfected with SMURF2 WT, SMURF2 DN, control siRNA, & SMURF2 siRNA (c). HUVECs transfected with control, SMURF2, or ZFYVE21 siRNA were treated with PRA & Rab5 co-IPs were performed (d). Recombinant FLAG-tagged ZFYVE21 (1 μg) and/or recombinant SMURF2 (1 μg) protein was incubated for 30 min at room temperature & analyzed by western blot (e). In vitro kinase assays were performed using various E2 ubiquitin ligases with SMURF2 protein (1 μg) as the E3 ubiquitin ligase & PTEN (1 μg) as substrate (f). In vitro kinase assays were performed using isolated SMURF2 WT or SMURF2 DN as E3 ubiquitin ligases, UbcH5c as the E2 ubiquitin ligase, & PTEN as substrate as indicated (g). HUVECs were pretreated with MG132 for 4 h or chloroquine for 30 min prior to PRA treatment at the indicated times prior to Rab5 co-IP & western blot analysis (h). Western blot analyses were repeated two to four times. Confocal microscopic studies analyzed ≥4 cells per group & was repeated three times. In vitro kinase assays were repeated twice. Representative data shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31113953), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00079038-b01p_mouse-polyclonal-zfyve21-antibody-310202415485117.jpg)
![Western Blot: ZFYVE21 Antibody [NBP2-82050] - Western blot analysis of ZFYVE21 in 3T3 cell tissue lysate with ZFYVE21 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-NBP2-82050-img0001.jpg "Western Blot: ZFYVE21 Antibody [NBP2-82050] - Western blot analysis of ZFYVE21 in 3T3 cell tissue lysate with ZFYVE21 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: ZFYVE21 Antibody - BSA Free [NBP2-82050] - Immunofluorescence of ZFYVE21 in human kidney tissue with ZFYVE21 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82050_rabbit-polyclonal-zfyve21-antibody-2711202420414546.jpg)
![Immunohistochemistry: ZFYVE21 Antibody [NBP2-30498] - Staining of human kidney shows cytoplasmic and extracellular positivity in glomeruli.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Immunohistochemistry-NBP2-30498-img0001.jpg "Immunohistochemistry: ZFYVE21 Antibody [NBP2-30498] - Staining of human kidney shows cytoplasmic and extracellular positivity in glomeruli.")
![Western Blot: ZFYVE21 Antibody [NBP1-98434] - HCT15 Cell Lysate 1.0 ug/ml, Gel Concentration: 12%](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-Western-Blot-NBP1-98434-img0002.jpg "Western Blot: ZFYVE21 Antibody [NBP1-98434] - HCT15 Cell Lysate 1.0 ug/ml, Gel Concentration: 12%")
![Western Blot: ZFYVE21 Antibody (2B12) [NBP2-46407] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFYVE21.](http://images.novusbio.com/fullsize/ZFYVE21-Antibody-2B12-Western-Blot-NBP2-46407-img0001.jpg "Western Blot: ZFYVE21 Antibody (2B12) [NBP2-46407] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFYVE21.")