VEGF Antibodies

1603-CV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine VEGF (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine VEGF₁₆₄Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603). The ND₅₀ is typically 0.04-0.10 µg/mL." class="big_lightbox"> 1603-CV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine VEGF (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine VEGF₁₆₄Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603). The ND₅₀ is typically 0.04-0.10 µg/mL." alt="Recombinant Canine VEGF (Catalog # 1603-CV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine VEGF (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine VEGF₁₆₄Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603). The ND₅₀ is typically 0.04-0.10 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1603-CV) was serially diluted and captured by Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1603-CV) was serially diluted and captured by Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1603-CV) was serially diluted and captured by Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1603-CV) was serially diluted and captured by Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

564-RV) was serially diluted and captured by Mouse Anti-Rat VEGF 164 Monoclonal Antibody (Catalog # MAB564) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 564-RV) was serially diluted and captured by Mouse Anti-Rat VEGF 164 Monoclonal Antibody (Catalog # MAB564) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Rat VEGF 164 (Catalog # 564-RV) was serially diluted and captured by Mouse Anti-Rat VEGF 164 Monoclonal Antibody (Catalog # MAB564) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF₁₆₅(100 ng/mL) is neutral­lized (green line) by increasing concentrations of Mouse Anti-Zebrafish VEGF Monoclonal Antibody (Catalog # MAB1247). The ND₅₀ is typically 0.2-0.5 µg/mL." class="big_lightbox"> 1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF₁₆₅(100 ng/mL) is neutral­lized (green line) by increasing concentrations of Mouse Anti-Zebrafish VEGF Monoclonal Antibody (Catalog # MAB1247). The ND₅₀ is typically 0.2-0.5 µg/mL." alt="Recombinant Zebrafish VEGF₁₆₅(Catalog # 1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF₁₆₅(100 ng/mL) is neutral­lized (green line) by increasing concentrations of Mouse Anti-Zebrafish VEGF Monoclonal Antibody (Catalog # MAB1247). The ND₅₀ is typically 0.2-0.5 µg/mL." class="big_thumb" />

293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF₁₆₅(10 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human VEGF (Bevacizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9947). The ND₅₀ is typically 10-80 ng/mL." class="big_lightbox"> 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF₁₆₅(10 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human VEGF (Bevacizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9947). The ND₅₀ is typically 10-80 ng/mL." alt="Recombinant Human VEGF₁₆₅(Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF₁₆₅(10 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human VEGF (Bevacizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9947). The ND₅₀ is typically 10-80 ng/mL." class="big_thumb" />

1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF₁₆₅(80 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Zebrafish VEGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1247). The ND₅₀ is typically 0.3-0.6 µg/mL." class="big_lightbox"> 1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF₁₆₅(80 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Zebrafish VEGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1247). The ND₅₀ is typically 0.3-0.6 µg/mL." alt="Recombinant Zebrafish VEGF₁₆₅(Catalog # 1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF₁₆₅(80 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Zebrafish VEGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1247). The ND₅₀ is typically 0.3-0.6 µg/mL." class="big_thumb" />