VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Tau phosphorylated at T231 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Phospho-Tau (T231) Monoclonal Antibody (Catalog # MAB34941) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB34941-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82247-0.025ml | |||
CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Tau was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Tau Monoclonal Antibody (Catalog # MAB3494) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3494-SP | |||
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons and neuronal processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Tau was detected in immersion fixed paraffin-embedded sections of human brain (Alzheimer's) using Mouse Anti-Human Tau Monoclonal Antibody (Catalog # MAB106291) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons and neuronal processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB106291-SP | |||
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Tau was detected in immersion fixed paraffin-embedded sections of human brain (Alzheimer's) using Mouse Anti-Human Tau Monoclonal Antibody (Catalog # MAB10629) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10629-SP | |||
![Western Blot: Tau Antibody [NB100-82247] - Analysis of extracts from mouse brain tissue.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NB100-82247-img0005.jpg "Western Blot: Tau Antibody [NB100-82247] - Analysis of extracts from mouse brain tissue.")
![Immunohistochemistry-Paraffin: Tau Antibody [NB100-82247] - Analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer's Disease using NB100-82247](http://images.novusbio.com/fullsize/Tau-Antibody-Immunohistochemistry-NB100-82247-img0003.jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025124758..jpg "<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein by immunofluorescent analysis.<br>Sample: DIV11 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.<br>Red: MAP2, a microtubule marker, stained by MAP2 antibody [HM-2] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-immunohistochemistry-3042025123354..jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein at cytoskeleton by immunofluorescent analysis. <br>Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.<br>Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.<br>Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-immunocytochemistry-immunofluorescence-3042025125627..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Untreated (–) and treated (+) mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025125836..jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513045..jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513345..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513540..jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025132854..jpg)
![<b>Orthogonal Strategies Validation. </b>Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] (NBP3-45160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-141120251831211.jpg)
![Tau antibody [HL2736] detects Tau protein by immunohistochemical analysis.</br>Sample: Frozen-sectioned mouse eye.</br>Green: Tau stained by Tau antibody [HL2736] (NBP3-45160) diluted at 1:250.</br>Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunohistochemistry-frozen-14112025185543.jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-294202517153..jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Untreated (–) and treated (+) mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (phospho Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-30420259378..jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Whole cell and rat tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2497] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-western-blot-304202593920..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.<br><br><strong>Capture:</strong> Tau (p Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)<br><strong>Detection:</strong> HRP-conjugated Tau (p Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)<br>Please notice that NBP3-45157 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45158.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202594949.jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2497) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.<br><br><strong>Capture:</strong> Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)<br><strong>Detection:</strong> HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)Please notice that NBP3-45158 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-45157. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-elisa-304202512432..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2497) [NBP3-45157] - Tau (p Thr181) antibody [HL2497] detects Tau (p Thr181) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).<br>Sample: Paraffin-embedded mouse brain.<br>Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2497] (GTX638855) diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45157_rabbit-tau-p-thr181-mab-hl2497-azide-and-bsa-free-immunohistochemistry-3042025122656..jpg)
![ELISA: Tau [p Thr217] Antibody [NBP3-13481] - Indirect ELISA analysis performed by coating plate with recombinant partial Tau peptide and Tau (phospho Thr217) peptide (1000-0.003 ng/mL). Coated protein was probed with Tau (phospho Thr217) antibody (NBP3-13481) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.](http://images.novusbio.com/fullsize/Tau-[p-Thr217]-Antibody-ELISA-NBP3-13481-img0002.jpg "ELISA: Tau [p Thr217] Antibody [NBP3-13481] - Indirect ELISA analysis performed by coating plate with recombinant partial Tau peptide and Tau (phospho Thr217) peptide (1000-0.003 ng/mL). Coated protein was probed with Tau (phospho Thr217) antibody (NBP3-13481) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (NBP2-19301) (1:10000) was used to detect bound primary antibody.")
![IHC-P-Tau [p Thr217] Antibody-Rat brain. Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp3-13481_rabbit-tau-p-thr-217-pab-immunohistochemistry-paraffin-89202384342..jpg)
![Tau (phospho Thr217) antibody detects Tau (phospho Thr217) protein at nucleus and cytoskeleton by immunofluorescent analysis.</br>Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: Tau (phospho Thr217) stained by Tau (phospho Thr217) antibody (NBP3-13481) diluted at 1:250.</br>Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13481_rabbit-tau-p-thr-217-pab-immunocytochemistry-immunofluorescence-2611202518274612.jpg)
![Immunohistochemistry-Paraffin-NBP3-21454-Tau [p Ser396] Antibody (SN62-09)-Rat brain tissue using NBP3-21454. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-immunohistochemistry-paraffin-16820239811.jpg "Immunohistochemistry-Paraffin-NBP3-21454-Tau [p Ser396] Antibody (SN62-09)-Rat brain tissue using NBP3-21454. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Mouse brain tissue using NBP3-21454. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-immunohistochemistry-paraffin-16820239936.jpg)
![Immunocytochemistry/ Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Immunocytochemical analysis of Tau [p Ser396] in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-103202523464548.jpg)
![Immunocytochemistry/ Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP3-21454] - Immunocytochemical analysis of Tau [p Ser396] in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/nbp3-21454_rabbit-tau-p-ser396-mab-sn62-09-103202523464554.jpg)
![Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-western-blot-152025102720.jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Untreated (–) and treated (+) Mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau (p Thr181) antibody [HL2498] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-western-blot-152025103434..jpg)
![Immunohistochemistry: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Tau (p Thr181) antibody [HL2498] detects Tau (phospho Thr181) protein in the cytoplasm by immunohistochemical analysis in the presence of non phospho control peptide (left) but not in the presence of the antigenic phospho peptide (right).<br>Sample: Paraffin-embedded mouse brain.<br>Tau (p Thr181) stained by Tau (p Thr181) antibody [HL2498] diluted at 1:100.](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-immunohistochemistry-152025103033..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2498) [NBP3-45158] - Sandwich ELISA detection of Tau peptide and Tau (phospho Thr181) peptide using antibodies as below.<br><br><strong>Capture:</strong> Tau (phospho Thr181) antibody [HL2498] (NBP3-45158) (5 ug/mL)<br><strong>Detection:</strong> HRP-conjugated Tau (phospho Thr181) antibody [HL2497] (NBP3-45157) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-elisa-152025104331..jpg)
![ELISA: Rabbit Tau [p Thr181] mAb (HL2498) - Sandwich ELISA detection of Tau peptide and Tau (p Thr181) peptide using antibodies as below.<br><br><strong>Capture:</strong> Tau (p Thr181) antibody [HL2497] (NBP3-45157) (5 ug/mL)<br><strong>Detection:</strong> HRP-conjugated Tau (p Thr181) antibody [HL2498] (NBP3-45158) (1 ug/mL)](http://images.novusbio.com/fullsize/nbp3-45158_rabbit-tau-p-thr181-mab-hl2498-azide-and-bsa-free-elisa-152025105927..jpg)
![Western Blot: Tau Antibody (1D5) - Pre-formed Fibrils [NBP2-79825] - Western Blot analysis of Human Breast Cancer Cell line and Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (NBP2-79825). Lane 1: MW Marker. Lane 2: Human T-47d (10ug). Lane 3: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (NBP2-79825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize/Tau-Antibody-1D5-Pre-formed-Fibrils-Western-Blot-NBP2-79825-img0004.jpg "Western Blot: Tau Antibody (1D5) - Pre-formed Fibrils [NBP2-79825] - Western Blot analysis of Human Breast Cancer Cell line and Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (NBP2-79825). Lane 1: MW Marker. Lane 2: Human T-47d (10ug). Lane 3: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (NBP2-79825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![Dot Blot: Tau Antibody (1D5) - Pre-formed Fibrils [NBP2-79825] - Dot Blot analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5 (NBP2-79825). Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (NBP2-79825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking.](http://images.novusbio.com/fullsize/Tau-Antibody-1D5-Pre-formed-Fibrils-Dot-Blot-NBP2-79825-img0005.jpg)
![Simple Western: Tau [p Ser262] Antibody [NB100-82242] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.](http://images.novusbio.com/fullsize/Tau-[p-Ser262]-Antibody-Simple-Western-NB100-82242-img0004.jpg "Simple Western: Tau [p Ser262] Antibody [NB100-82242] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.")
![Western Blot: Tau [p Ser262] Antibody [NB100-82242] - Western blot analysis of lysates from HeLa cells, using NB100-82242. The lane on the left is blocked with the phospho peptide.](http://images.novusbio.com/fullsize/nb100-82242_rabbit-polyclonal-tau-p-ser262-antibody-234202415453510.jpg)
