PDGF-C Antibodies

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="PDGF‑C was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse PDGF‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1447) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF109). Specific bands were detected for PDGF-C p80 at approximately 80 kDa and PDGF-C p55 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF109). Specific bands were detected for PDGF-C p80 at approximately 80 kDa and PDGF-C p55 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for PDGF-C p80 at approximately 80 kDa and PDGF-C p55 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

1687-CC) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-CC (0.8 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560). The ND₅₀ is typically 6-24 µg/mL." class="big_lightbox"> 1687-CC) stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-CC (0.8 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1560). The ND₅₀ is typically 6-24 µg/mL." class="big_thumb" />

1687-CC) induces proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human PDGF-CC (1 ug/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF-C Monoclonal Antibody (Catalog # MAB1560). The ND₅₀ is typically 0.6-3 ug/ml." class="big_lightbox"> 1687-CC) induces proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human PDGF-CC (1 ug/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF-C Monoclonal Antibody (Catalog # MAB1560). The ND₅₀ is typically 0.6-3 ug/ml." alt="Recombinant Human PDGF-CC (Catalog # 1687-CC) induces proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human PDGF-CC (1 ug/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human PDGF-C Monoclonal Antibody (Catalog # MAB1560). The ND₅₀ is typically 0.6-3 ug/ml." class="big_thumb" />