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Neuregulin-1 Isoform SMDF Antibodies

We offer Neuregulin-1 Isoform SMDF Antibodies for use in common research applications: Neutralization, Western Blot. Each Neuregulin-1 Isoform SMDF Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Neuregulin-1 Isoform SMDF Antibodies can be used in a variety of model species: Human. Use the list below to choose the Neuregulin-1 Isoform SMDF Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Neuregulin-1 Isoform SMDF polyclonal antibodies.

Alternate Names for Neuregulin-1 Isoform SMDF Antibodies

anti-Neuregulin-1 Isoform SMDF antibody, anti-NRG1 antibody

5 Results for "neuregulin-1-isoform-smdf" in Primary Antibodies

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AF378

Neuregulin-1 Isoform SMDF Antibody

100 μg

AF378

Recombinant Human NRG1 Isoform SMDF (Catalog # <a class=

378-SM) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1 Isoform SMDF (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human NRG1 Isoform SMDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF378). The ND₅₀ is typically 2-8 µg/mL." alt="Recombinant Human NRG1 Isoform SMDF (Catalog # 378-SM) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1 Isoform SMDF (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human NRG1 Isoform SMDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF378). The ND₅₀ is typically 2-8 µg/mL." class="big_thumb" />

SMDF transforms via ErbB receptor signalling.(A) Western blot analysis of indicated proteins in NSLT cells expressing SMDF, SMDF* (point mutant incapable of binding ErbB receptors) or control vector (LXSN), and SMDF(T) cells derived from SMDF tumours in the absence or presence of the ErbB-2 inhibitor, 4557W (40 nM). (B) Cell counts of triplicate wells in the presence or absence of the indicated concentrations of 4557W −/+ S.D.. Figure is representative of 3 independent experiments. (C) Number of colonies formed in soft agar assays of NSLT cells expressing SMDF, Ras or control vector LXSN in the absence or presence of the indicated inhibitors. U0126 (UO), LY294002 (LY) and the ErbB inhibitor (ErbBI). 12 fields were counted per well with the results shown as average/well −/+ S.D.. Results are representative of two separate experiments (D) Soft agar assays of NSLT cells infected with SMDF, SMDF* or control vector (E) 105 NSLT cells expressing SMDF, Ras or control vector (LXSN) were seeded in triplicate into 6 well dishes and counted at 72 h. The inhibitors U0126 (20 µM), LY294002 (20 µM) or control vehicle were added where indicated at 36 hours. Western blot analysis of total lysates from NSLT cells expressing SMDF or control vector (LXSN). (F) Western blot analysis of total lysates from NSLT cells expressing SMDF or control vector (LXSN) collected 3 (preconfluent), 6 and 9 days after seeding. Plates were medium changed daily. (G) Western blot analysis of total lysates from NSLT cells expressing SMDF, Ras or control vector (LXSN) in attached (att) or anchorage-independent conditions (sus). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20668675), licensed under a CC-BY license. Not internally tested by R&D Systems.

SMDF promotes oncogenic transformation.(A) Western blot analysis of total lysates from NSLT cells selected to express human SMDF or control vector (LXSN). SMDF(T) are derived from tumours, induced by injecting NSLTSMDF cells into nude mice. (B) Cell counts −/+ S.D. following seeding of 105 NSLT cells per well expressing SMDF, Ras or control vector (LXSN) in triplicate in 6 well plates. Medium was changed daily. Experiment shown is representative of 3 separate experiments. Representative phase-contrast images of the cells at high density. (C) NSLT cells expressing SMDF, Ras or control vector (LXSN) were injected into the flanks of nude mice. Ras cells were only injected into a single flank. Picture shows examples of tumours formed (SMDF (left), Ras (right)) and the graph shows the average growth of the tumours −/+ S.D.. (D) Representative images of cells dissociated from NSLTSMDF derived tumours stained for LT (upper panel) or the Schwann cell marker, S100 (lower panel). Primary Schwann cells (NS) were infected with retroviral vectors expressing SMDF, Ras or the control vector (LXSN) and selected in G418. One week following selection, the cultures were fixed and stained for the senescence marker beta -Galactosidase at pH 6 (E) or seeded in triplicate and counted for four passages (F). Western blot shows p-ERK levels in lysates prepared from NS cells expressing Ras, SMDF or control vector (LXSN) six days after infection. (G) 2×105 NS cells infected with SMDF or control vector (LXSN) were plated in triplicate in 6 well dishes and counted at the indicated days. Medium was changed daily. Error bars show −/+ S.D.. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20668675), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF378U-100UG
Alexa Fluor 405	AF378V-100UG
Alexa Fluor 488	AF378G-100UG
Alexa Fluor 532	AF378X-100UG
Alexa Fluor 594	AF378T-100UG
Alexa Fluor 647	AF378R-100UG
Alexa Fluor 700	AF378N-100UG
Alexa Fluor 750	AF378S-100UG
Alexa Fluor Plus 405	AF378AFP405-100UG
Alexa Fluor Plus 488	AF378AFP488-100UG
Alexa Fluor Plus 555	AF378AFP555-100UG
Alexa Fluor Plus 594	AF378AFP594-100UG
Alexa Fluor Plus 647	AF378AFP647-100UG
Alexa Fluor Plus 680	AF378AFP680-100UG
Allophycocyanin	AF378A
Biotin	BAF378
CoraFluor 1	AF378CL1
DyLight 350	AF378D
DyLight 405	AF378E
DyLight 488	AF378K
DyLight 550	AF378L
DyLight 594	AF378M
DyLight 650	AF378W
DyLight 680	AF378Y
DyLight 755	AF378Z
FITC	AF378F
HRP	AF378H
Janelia Fluor 525	AF378JF525
Janelia Fluor 549	AF378I
Janelia Fluor 646	AF378J
Janelia Fluor 669	AF378JF669
PE	AF378P
PerCP	AF378C
mFluor Violet 450 SE	AF378MFV450
mFluor Violet 500 SE	AF378MFV500
mFluor Violet 610 SE	AF378MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF378-SP

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AF378AFP647

Neuregulin-1 Isoform SMDF Antibody [Alexa Fluor™ Plus 647]

100 μg

Reactivity:

Species Glossary

Reactivity: Hu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 647

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AF378AFP405

Neuregulin-1 Isoform SMDF Antibody [Alexa Fluor™ Plus 405]

100 μg

Reactivity:

Species Glossary

Reactivity: Hu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 405

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AF378AFP488

Neuregulin-1 Isoform SMDF Antibody [Alexa Fluor™ Plus 488]

100 μg

Reactivity:

Species Glossary

Reactivity: Hu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 488

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AF378AFP680

Neuregulin-1 Isoform SMDF Antibody [Alexa Fluor™ Plus 680]

100 μg

Reactivity:

Species Glossary

Reactivity: Hu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 680

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

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Reactivity

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Available Conjugates

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Species Glossary

Neuregulin-1 Isoform SMDF Antibodies

Neuregulin-1 Isoform SMDF Antibody

Neuregulin-1 Isoform SMDF Antibody [Alexa Fluor™ Plus 647]

Neuregulin-1 Isoform SMDF Antibody [Alexa Fluor™ Plus 405]

Neuregulin-1 Isoform SMDF Antibody [Alexa Fluor™ Plus 488]

Neuregulin-1 Isoform SMDF Antibody [Alexa Fluor™ Plus 680]