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![Immunocytochemistry/ Immunofluorescence: IRX4 Antibody [NBP3-23509] - Immunofluorescence staining of IRX4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-23509 (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-23509_rabbit-irx4-pab-235202514331515.png "Immunocytochemistry/ Immunofluorescence: IRX4 Antibody [NBP3-23509] - Immunofluorescence staining of IRX4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-23509 (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Nucleus.")