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![Immunohistochemistry: Insulin B chain Antibody [NBP3-33144] - Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Insulin B chain antibody (NBP3-33144) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33144) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33144_rabbit-insulin-b-chain-pab-29820241420949.jpg "Immunohistochemistry: Insulin B chain Antibody [NBP3-33144] - Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Insulin B chain antibody (NBP3-33144) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33144) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: Insulin B chain Antibody [NBP3-33144] - Flow cytometric analysis of Insulin B chain was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-33144, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).](http://images.novusbio.com/fullsize/nbp3-33144_rabbit-insulin-b-chain-pab-29202416453245.jpg)