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![Non-transfected and transfected 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (NBP3-25375) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25375_rabbit-influenza-a-h5n8-hemagglutinin-mab-hl1550-azide-bsa-free-western-blot-261120251826131.jpg "Non-transfected and transfected 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (NBP3-25375) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, full-length Avian Influenza A virus HSN8 HA (Hemagglutinin) protein (63.49-0.99 nM). Coated protein was probed with Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (NBP3-25375) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25375_rabbit-influenza-a-h5n8-hemagglutinin-mab-hl1550-azide-bsa-free-elisa-26112025183963.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] (NBP3-25375) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25375_rabbit-influenza-a-h5n8-hemagglutinin-mab-hl1550-azide-bsa-free-western-blot-26112025197183.jpg)