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![Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-012-Immunocytochemistry-Immunofluorescence-NBP2-89306-img0001.jpg "Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Staining of Human IGF1R in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human IGF1R monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.")
![Flow Cytometry: IGF-I R/IGF1R Antibody (012) [NBP2-89306] - Analysis of human IGF1R expression on HeLa cells. Cells were stained with purified anti-Human IGF1R, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-Antibody-012-Flow-Cytometry-NBP2-89306-img0002.jpg)
![Western Blot: IGF-I R/IGF1R [p Tyr1146] Antibody [NBP3-13236] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (NBP3-13236) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-[p-Tyr1146]-Antibody-Western-Blot-NBP3-13236-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: IGF-I R/IGF1R [p Tyr1146] Antibody [NBP3-13236] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF1R (phospho Tyr1131) / Insulin Receptor (phospho Tyr1146) antibody (NBP3-13236) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: IGF-I R/IGF1R [p Tyr950] Antibody [NBP3-13235] - Untreated (-) and treated (+) MCF-7 whole cell extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF-I R/IGF1R (phospho Tyr980) antibody (NBP3-13235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IGF-I-R-IGF1R-[p-Tyr950]-Antibody-Western-Blot-NBP3-13235-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: IGF-I R/IGF1R [p Tyr950] Antibody [NBP3-13235] - Untreated (-) and treated (+) MCF-7 whole cell extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IGF-I R/IGF1R (phospho Tyr980) antibody (NBP3-13235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
