HSP27 Antibodies
We offer HSP27 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each HSP27 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HSP27 Antibodies can be used in a variety of model species: Amphibian, Avian, Bovine, Canine, Chicken, Equine, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Sheep. Use the list below to choose the HSP27 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HSP27 polyclonal antibodies and browse our HSP27 monoclonal antibody catalog.
Alternate Names for HSP27 Antibodies
anti-HSP27 antibody, anti-HSPB1 antibody, anti-28 kDa heat shock protein antibody, anti-DKFZp586P1322 antibody, anti-Estrogen-regulated 24 kDa protein antibody, anti-Heat shock 27 kDa protein antibody, anti-heat shock 27kD protein 1 antibody, anti-heat shock 27kDa protein 1 antibody, anti-heat shock protein beta-1 antibody, anti-HMN2B antibody, anti-HS.76067 antibody, anti-Hsp25 antibody, anti-HSP27HSP 27 antibody, anti-HSP28CMT2F antibody, anti-SRP27 antibody, anti-Stress-responsive protein 27 antibody
42 Results for "hsp27" in Primary Antibodies
HAF008). A specific band was detected for Phospho-HSP27 (S78/S82) at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-HSP27 (S78/S82) Monoclonal Antibody (Catalog # MAB23141) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-HSP27 (S78/S82) at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
HAF005). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human HSP27 Monoclonal Antibody (Catalog # MAB15801) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
HAF005). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line untreated (-) or treated (+) with 20 mJ/cm2ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.25 µg/mL of Rat Anti-Human HSP27 (S78/S82) Monoclonal Antibody (Catalog # MAB23142) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for 
NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in epithelial cells. View our protocol for ![Western Blot: HSP27 Antibody (5D7) [NBP2-37599] - Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate.](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-Western-Blot-NBP2-37599-img0002.jpg "Western Blot: HSP27 Antibody (5D7) [NBP2-37599] - Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (5D7) [NBP2-37599] - Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-Immunocytochemistry-Immunofluorescence-NBP2-37599-img0003.jpg)
![Immunohistochemistry: HSP27 Antibody (5D7) [NBP2-37599] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-Immunohistochemistry-NBP2-37599-img0006.jpg)
![Flow Cytometry: HSP27 Antibody (5D7) [NBP2-37599] - Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-Flow-Cytometry-NBP2-37599-img0004.jpg)
![Immunohistochemistry: HSP27 Antibody (5D7) [NBP2-37599] - Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-Immunohistochemistry-NBP2-37599-img0005.jpg)
![ELISA: HSP27 Antibody (5D7) [NBP2-37599] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/HSP27-Antibody-5D7-ELISA-NBP2-37599-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody (967050) [MAB1580] - HSP-27 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using HSP-27 Monoclonal Antibody at 5 ug/ml. Tissue was stained using VC001 (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.](http://images.novusbio.com/fullsize/HSP27-Antibody-967050-Immunohistochemistry-Paraffin-MAB1580-img0001.jpg "Immunohistochemistry-Paraffin: HSP27 Antibody (967050) [MAB1580] - HSP-27 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using HSP-27 Monoclonal Antibody at 5 ug/ml. Tissue was stained using VC001 (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.")
![Simple Western: HSP27 Antibody (967050) [MAB1580] - MCF-7 cell lysate. Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/HSP27-Antibody-967050-Simple-Western-MAB1580-img0002.jpg)
![Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Western-Blot-MAB9355-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions")
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - An intracellular stain was performed on MCF7 cells with HSP27 [p Ser78] Antibody MAB9355 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Flow-Cytometry-MAB9355-img0003.jpg)
![Simple Western: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Lane vies shows a specific band for HSP27 using 0.2 mg/mL MCF-7 -/+ UV cell lysates and antibody at 5 ug/mL. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 32 kDa. Image reported by internal validation.](http://images.novusbio.com/fullsize/HSP27-p-Ser78-Antibody-2079A-Simple-Western-MAB9355-img0004.jpg)
![Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-25150-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2000 of Chicken Anti-Human HSP29 Polyclonal Antibody (Catalog # NBP2-25150) followed by HRP-conjugated Anti-Chicken Secondary Antibody. Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-25150] - Analysis of whole cell lysates using chicken pAb to HSP27, NBP2-25150, dilution 1:2,000, in red. [1] protein standard (red), [2] NIH-3T3 , [3] C6, [4] HeLa, [5] HEK293, [6] SH-SY5Y, and [7] COS-1 cells. Strong single band at 27kDa corresponds to the HSP27 protein strongly expressed in human cell lines. Note that the antibody does not recognize rodent HSP27. The blot was simultaneously probed with mouse mAb to Beta-tubulin, dilution 1:10,000 in green. A single band at 50kDa, which is detected in all cell lysates, corresponds to the Beta-tubulin proteins.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP2-25150] - Staining of HeLa cells stained with chicken pAb to heat shock protein 27 (HSP27), NBP2-25150, dilution 1:1,000 in green, and costained with mouse mAb to HSP60, dilution 1:5,000 in red. Blue is DAPI staining of nuclear DNA. NBP2-25150 antibody produces strong cytoplasmic staining, while the HSP60 antibody specifically labels mitochondria.](http://images.novusbio.com/fullsize/HSP27-Antibody-NBP2-25150-img0001.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NBP1-89666-img0009.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NBP1-89666-img0010.jpg)
![Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Western-Blot-NBP3-13617-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - HSP27 (phospho Ser82) antibody [GT357] detects HSP27 (phospho Ser82) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP27 (phospho Ser82) stained by HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Immunocytochemistry-Immunofluorescence-NBP3-13617-img0002.jpg)
![Western Blot: HSP27 [p Ser82] Antibody (GT357) [NBP3-13617] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-p-Ser82-Antibody-GT357-Western-Blot-NBP3-13617-img0003.jpg)
![Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody [GT357] (NBP3-13617) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13617_mouse-hsp27-p-ser-82-mab-gt357-western-blot-2611202518484816.jpg)
![Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/HSP27-[p-Ser82]-Antibody-Western-Blot-NBP3-13297-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-[p-Ser82]-Antibody-Western-Blot-NBP3-13297-img0001.jpg)
![Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/HSP27-[p-Ser82]-Antibody-Western-Blot-NBP3-13297-img0002.jpg)
