GPR75 Antibodies

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="GPR75 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human GPR75 Monoclonal Antibody (Catalog # MAB6728) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol. " alt="HEK293 human embryonic kidney cell line transfected with (A) human GPR75 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR75 Monoclonal Antibody (Catalog # MAB67281) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />