FCHSD1 Antibodies
We offer FCHSD1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each FCHSD1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our FCHSD1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the FCHSD1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our FCHSD1 polyclonal antibodies and browse our FCHSD1 monoclonal antibody catalog.
Alternate Names for FCHSD1 Antibodies
anti-FCHSD1 antibody, anti-FCH and double SH3 domains 1 antibody, anti-FCH and double SH3 domains protein 1 antibody, anti-FLJ00007 antibody, anti-nervous wreck homolog 2 antibody, anti-NWK2 antibody
10 Results for "fchsd1" in Primary Antibodies
![Western Blot: FCHSD1 Antibody (4G4) [H00089848-M01] - Total proteins of postnatal day 5 mouse cochlea and vestibula were extracted and separated by PAGE and detected with antibodies against FCHSD1 (Novus), FCHSD2, or SNX9. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056516), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-4G4-Western-Blot-H00089848-M01-img0002.jpg "Western Blot: FCHSD1 Antibody (4G4) [H00089848-M01] - Total proteins of postnatal day 5 mouse cochlea and vestibula were extracted and separated by PAGE and detected with antibodies against FCHSD1 (Novus), FCHSD2, or SNX9. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056516), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - SNX9 colocalizes with FCHSD1 in the cuticular plate of mouse cochlear hair cells. Shown are single confocal sections. SNX9 immunoreactivity visualized with FITC-conjugated secondary antibody was distinctly associated with the cuticular plate, which was visualized with rhodamine-conjugated phalloidin. SNX9 immunoreactivity in the cuticular plate of 2-week old mouse cochlear hair cells. SNX9 immunoreactivity colocalized with FCHSD1 immunoreactivity, which was visualized with Cy5-conjugated secondary antibody, in the cuticular plate of 3 week-old mouse cochlear hair cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056516), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-4G4-Immunocytochemistry-Immunofluorescence-H00089848-M01-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - FCHSD1 and FCHSD2 immunolocalization in mouse cochlear hair cells. Shown are single confocal sections. FCHSD1 or FCHSD2 immunoreactivity visualized with Cy5 or FITC-conjugated secondary antibody was distinctly associated with stereocilia or cuticular plate, which were visualized with rhodamine-conjugated phalloidin. FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse cochlear hair cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056516), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-4G4-Immunocytochemistry-Immunofluorescence-H00089848-M01-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - FCHSD1 and FCHSD2 immunolocalization in mouse cochlear hair cells. Shown are single confocal sections. FCHSD1 or FCHSD2 immunoreactivity visualized with Cy5 or FITC-conjugated secondary antibody was distinctly associated with stereocilia or cuticular plate, which were visualized with rhodamine-conjugated phalloidin. FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056516), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-4G4-Immunocytochemistry-Immunofluorescence-H00089848-M01-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - SNX9 colocalizes with FCHSD1 in the cuticular plate of mouse cochlear hair cells. Shown are single confocal sections. SNX9 immunoreactivity visualized with FITC-conjugated secondary antibody was distinctly associated with the cuticular plate, which was visualized with rhodamine-conjugated phalloidin. SNX9 immunoreactivity colocalized with FCHSD1 immunoreactivity, which was visualized with Cy5-conjugated secondary antibody, in the cuticular plate of 3 week-old mouse cochlear hair cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056516), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-4G4-Immunocytochemistry-Immunofluorescence-H00089848-M01-img0006.jpg)
![Western Blot: FCHSD1 Antibody (4G4) [H00089848-M01] - Expression analysis of mouse Fchsd1 & Fchsd2 in different tissues.(A) Total RNA from postnatal day 5 mouse tissues was extracted & used as template for reverse transcription. PCR was performed using this cDNA as template. Upper panel, Fchsd1 mRNA is expressed more abundantly in nervous tissues. Middle panel, Fchsd2 mRNA is expressed ubiquitously in all tissues examined. Lower panel, beta -actin specific primers were used as the RT-PCR template control. (B) Total proteins of postnatal day 5 mouse cochlea & vestibula were extracted & separated by PAGE & detected with antibodies against FCHSD1(Novus), FCHSD2, or SNX9. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00089848-m01_mouse-monoclonal-fchsd1-antibody-4g4-310202415384148.jpg)
![Western Blot: FCHSD1 Antibody (4G4) [H00089848-M01] - Expression analysis of mouse Fchsd1 & Fchsd2 in different tissues.(A) Total RNA from postnatal day 5 mouse tissues was extracted & used as template for reverse transcription. PCR was performed using this cDNA as template. Upper panel, Fchsd1 mRNA is expressed more abundantly in nervous tissues. Middle panel, Fchsd2 mRNA is expressed ubiquitously in all tissues examined. Lower panel, beta -actin specific primers were used as the RT-PCR template control. (B) Total proteins of postnatal day 5 mouse cochlea & vestibula were extracted & separated by PAGE & detected with antibodies against FCHSD1(Novus), FCHSD2, or SNX9. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00089848-m01_mouse-monoclonal-fchsd1-antibody-4g4-310202415293332.jpg)
![Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - SNX9 colocalizes with FCHSD1 in the cuticular plate of mouse cochlear hair cells.Shown are single confocal sections. SNX9 immunoreactivity visualized with FITC-conjugated secondary antibody was distinctly associated with the cuticular plate, which was visualized with rhodamine-conjugated phalloidin. (A) SNX9 immunoreactivity in the cuticular plate of 2-week old mouse cochlear hair cells. (B) SNX9 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) & (D) SNX9 immunoreactivity colocalized with FCHSD1 immunoreactivity, which was visualized with Cy5-conjugated secondary antibody, in the cuticular plate of 3 week-old mouse cochlear hair cells. The Novus FCHSD1 antibody was used in (C) & (D). Scale bars: 10 µm in (A–C), 5 µm in (D). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00089848-m01_mouse-monoclonal-fchsd1-antibody-4g4-31020241548360.jpg)
![Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - FCHSD1 & FCHSD2 immunolocalization in mouse cochlear hair cells.Shown are single confocal sections. FCHSD1 or FCHSD2 immunoreactivity visualized with Cy5 or FITC-conjugated secondary antibody was distinctly associated with stereocilia or cuticular plate, which were visualized with rhodamine-conjugated phalloidin. (A) FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse cochlear hair cells. (B) FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) FCHSD2 immunoreactivity in the hair bundles of 6-week old mouse cochlear hair cells. (D) FCHSD2 immunoreactivity in the hair bundles of 7-month old mouse inner hair cells. (E) FCHSD2 immunoreactivity in the hair bundles of 2-week old mouse outer hair cells. The Novus FCHSD1 antibody was used in (A) & (B). Scale bars: 10 µm in(A-C), 5 µm in (D) & (E). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00089848-m01_mouse-monoclonal-fchsd1-antibody-4g4-310202415485129.jpg)
![Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - SNX9 colocalizes with FCHSD1 in the cuticular plate of mouse cochlear hair cells.Shown are single confocal sections. SNX9 immunoreactivity visualized with FITC-conjugated secondary antibody was distinctly associated with the cuticular plate, which was visualized with rhodamine-conjugated phalloidin. (A) SNX9 immunoreactivity in the cuticular plate of 2-week old mouse cochlear hair cells. (B) SNX9 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) & (D) SNX9 immunoreactivity colocalized with FCHSD1 immunoreactivity, which was visualized with Cy5-conjugated secondary antibody, in the cuticular plate of 3 week-old mouse cochlear hair cells. The Novus FCHSD1 antibody was used in (C) & (D). Scale bars: 10 µm in (A–C), 5 µm in (D). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00089848-m01_mouse-monoclonal-fchsd1-antibody-4g4-310202415483628.jpg)
![Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - FCHSD1 & FCHSD2 immunolocalization in mouse cochlear hair cells.Shown are single confocal sections. FCHSD1 or FCHSD2 immunoreactivity visualized with Cy5 or FITC-conjugated secondary antibody was distinctly associated with stereocilia or cuticular plate, which were visualized with rhodamine-conjugated phalloidin. (A) FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse cochlear hair cells. (B) FCHSD1 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) FCHSD2 immunoreactivity in the hair bundles of 6-week old mouse cochlear hair cells. (D) FCHSD2 immunoreactivity in the hair bundles of 7-month old mouse inner hair cells. (E) FCHSD2 immunoreactivity in the hair bundles of 2-week old mouse outer hair cells. The Novus FCHSD1 antibody was used in (A) & (B). Scale bars: 10 µm in(A-C), 5 µm in (D) & (E). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00089848-m01_mouse-monoclonal-fchsd1-antibody-4g4-310202415482261.jpg)
![Immunocytochemistry/ Immunofluorescence: FCHSD1 Antibody (4G4) [H00089848-M01] - SNX9 colocalizes with FCHSD1 in the cuticular plate of mouse cochlear hair cells.Shown are single confocal sections. SNX9 immunoreactivity visualized with FITC-conjugated secondary antibody was distinctly associated with the cuticular plate, which was visualized with rhodamine-conjugated phalloidin. (A) SNX9 immunoreactivity in the cuticular plate of 2-week old mouse cochlear hair cells. (B) SNX9 immunoreactivity in the cuticular plate of 3-week old mouse outer hair cells. (C) & (D) SNX9 immunoreactivity colocalized with FCHSD1 immunoreactivity, which was visualized with Cy5-conjugated secondary antibody, in the cuticular plate of 3 week-old mouse cochlear hair cells. The Novus FCHSD1 antibody was used in (C) & (D). Scale bars: 10 µm in (A–C), 5 µm in (D). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23437151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00089848-m01_mouse-monoclonal-fchsd1-antibody-4g4-310202415482252.jpg)
![Western Blot: FCHSD1 Antibody [NBP3-35232] - Western blot analysis of various lysates using FCHSD1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15S.](http://images.novusbio.com/fullsize/nbp3-35232_rabbit-fchsd1-pab-1012202411535874.jpg "Western Blot: FCHSD1 Antibody [NBP3-35232] - Western blot analysis of various lysates using FCHSD1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15S.")
![Immunohistochemistry: FCHSD1 Antibody [NBP3-35232] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using FCHSD1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35232_rabbit-fchsd1-pab-10122024120697.jpg)
![Immunohistochemistry: FCHSD1 Antibody [NBP3-35232] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using FCHSD1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35232_rabbit-fchsd1-pab-1012202411531060.jpg)
![Western Blot: FCHSD1 Antibody [NBP3-12654] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/FCHSD1-Antibody-Western-Blot-NBP3-12654-img0001.jpg "Western Blot: FCHSD1 Antibody [NBP3-12654] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody [NBP3-12654] - Staining of FCHSD1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FCHSD1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12654-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody [NBP3-12654] - Staining of FCHSD1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12654 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-12654_rabbit-polyclonal-fchsd1-antibody-235202514331581.png)
![Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody [NBP1-91894] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-Immunofluorescence-NBP1-91894-img0003.jpg "Immunocytochemistry/Immunofluorescence: FCHSD1 Antibody [NBP1-91894] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: FCHSD1 Antibody [NBP1-91894] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-Immunohistochemistry-Paraffin-NBP1-91894-img0002.jpg)
![Western Blot: FCHSD1 Antibody [NBP2-41137] - Analysis of FCHSD1 in MCF7 cell lysate with FCHSD1 antibody at (A) 0.5 and (B)1 ug/mL.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-Western-Blot-NBP2-41137-img0002.jpg "Western Blot: FCHSD1 Antibody [NBP2-41137] - Analysis of FCHSD1 in MCF7 cell lysate with FCHSD1 antibody at (A) 0.5 and (B)1 ug/mL.")
![Western Blot: FCHSD1 Antibody [NBP2-41137] - Western blot analysis of FCHSD1 in MCF7 cell lysate with FCHSD1 antibody at (A) 0.5 and (B)1 ug/mL.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-Western-Blot-NBP2-41137-img0001.jpg)
![Western Blot: FCHSD1 Antibody [H00089848-B01P] - Analysis of FCHSD1 expression in Y-79.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-Western-Blot-H00089848-B01P-img0003.jpg "Western Blot: FCHSD1 Antibody [H00089848-B01P] - Analysis of FCHSD1 expression in Y-79.")
![Western Blot: FCHSD1 Antibody [H00089848-B01P] - Analysis of FCHSD1 expression in transfected 293T cell line by FCHSD1 polyclonal antibody. Lane 1: FCHSD1 transfected lysate(44.99 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FCHSD1-Antibody-Western-Blot-H00089848-B01P-img0004.jpg)
![Western Blot: FCHSD1 Antibody [NBP2-22338] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: NBP2-22338 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FCHSD1 was also immunoprecipitated by rabbit anti-FCHSD1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](http://images.novusbio.com/fullsize/FCHSD1-Antibody-Western-Blot-NBP2-22338-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: FCHSD1 Antibody [NBP2-22338] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: NBP2-22338 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FCHSD1 was also immunoprecipitated by rabbit anti-FCHSD1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).")