CD4 Antibodies

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CD4 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2597) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat spleen tissue and rat CD4⁺T Cells. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat CD4 Monoclonal Antibody (Catalog # MAB6439) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_lightbox"> 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." alt="Cotton rat splenocytes were stained with Sheep Anti-Cotton Rat CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6676, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127)." class="big_thumb" />

Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> Fluorescent ICC Staining of Non-adherent Cells." alt="CD4 was detected in immersion fixed non-stimulated feline peripheral blood mononuclear cells (PBMCs) using 25 µg/mL Mouse Anti-Feline CD4 Monoclonal Antibody (Catalog # MAB25971) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB37912). Cells were washed and stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog #MAB114341) or (B) Mouse IgG2B Isotype Control (MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC conjugated Monoclonal Antibody (FAB100A). This demonstrates non-competing epitopes of MAB114341 and MAB37912 which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB37912). Cells were washed and stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog #MAB114341) or (B) Mouse IgG2B Isotype Control (MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC conjugated Monoclonal Antibody (FAB100A). This demonstrates non-competing epitopes of MAB114341 and MAB37912 which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins. " alt="PBMC lymphocytes were stained with CD4 RPA-T4 (MAB37912). Cells were washed and stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog #MAB114341) or (B) Mouse IgG2B Isotype Control (MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B) and Mouse Anti-Human CD3 epsilon APC conjugated Monoclonal Antibody (FAB100A). This demonstrates non-competing epitopes of MAB114341 and MAB37912 which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

FAB114341P), and Mouse Anti-Human CD4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (FAB37912R). This demonstrates non-competing epitopes of the two clones, which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> FAB114341P), and Mouse Anti-Human CD4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (FAB37912R). This demonstrates non-competing epitopes of the two clones, which allows for purity analysis after CD4+ selection. View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

MAB003), followed by PE-conjugated anti-Mouse IgG secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB003), followed by PE-conjugated anti-Mouse IgG secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB37911) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003), followed by PE-conjugated anti-Mouse IgG secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="CD4 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB11436) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />