BCL-W Antibodies

HAF008). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="big_lightbox"> HAF008). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." alt="Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1.2 µg/mL Rabbit Anti-Human/Mouse Bcl-w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF824) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="big_thumb" />

HAF007). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9with 25 mM Tris and 0.15 M NaCl." class="big_lightbox"> HAF007). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9with 25 mM Tris and 0.15 M NaCl." alt="Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and CTLL-2 mouse cytotoxic T cell line. The PVDF membrane was probed with 1 µg/mL Human Bcl-w Monoclonal Antibody (Catalog # MAB824) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Bcl-w Minus C-Terminus (Catalog # 824-BW) (1 ng) was included. A specific band for Bcl-w was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9with 25 mM Tris and 0.15 M NaCl." class="big_thumb" />

CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Bcl‑w was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Bcl‑w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8241) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

882-B8) induced release of Cytochrome c from mitochondriaBID + Bcl-w: Recombinant Human Bcl-w (824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondriaBID + Bcl-w + MAB8241: Mouse Anti-Human/Mouse Bcl-w Monoclonal Antibody (Catalog # MAB8241) neutralized Recombinant Human Bcl-w (824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondria" class="big_lightbox"> 882-B8) induced release of Cytochrome c from mitochondriaBID + Bcl-w: Recombinant Human Bcl-w (824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondriaBID + Bcl-w + MAB8241: Mouse Anti-Human/Mouse Bcl-w Monoclonal Antibody (Catalog # MAB8241) neutralized Recombinant Human Bcl-w (824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondria" alt="Cytochrome c released from enriched mouse liver mitochondria solubilized with Triton^®X-100 was quantified with the Rat/Mouse Cytochrome c ELISA (Catalog # MCTC0).Untreated: Release of Cytochrome c from untreated mitochondriaBID: Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondriaBID + Bcl-w: Recombinant Human Bcl-w (824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondriaBID + Bcl-w + MAB8241: Mouse Anti-Human/Mouse Bcl-w Monoclonal Antibody (Catalog # MAB8241) neutralized Recombinant Human Bcl-w (824-BW) inhibition of Recombinant Human BID (Caspase-8-cleaved) (882-B8) induced release of Cytochrome c from mitochondria" class="big_thumb" />